STUDIES OF ERYTHROCYTE MEMBRANE BAND 3

红细胞膜带 3 的研究

• 批准号: 3272271
• 负责人: PHILIP Stewart LOW
• 金额: $ 13.07万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-07-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3272271
• 关键词: X ray crystallography; affinity chromatography; ankyrins; band 3 protein; binding proteins; blood preservation; cell age; chemical binding; crystallization; cytoskeleton; dogs; epitope mapping; erythrocyte membrane; erythrocytes; fluorescence microscopy; human tissue; phosphatidylinositols; phosphorylation; protein structure function; proteolysis; sickle cell anemia; site directed mutagenesis; transport proteins; western blottings

The red cell membrane is often used as a model of other biological membranes, since i) it is abundantly available in highly purified form, ii) it can be obtained from humans without harming the donor, iii) its structure is comparatively simple, iv) the major components are found in virtually all animal cell plasma membranes, and v) pathological lesions arising in other cell membranes are often manifested in the red cell (e.g. diabetes, muscular dystrophy, etc.). For this reason, a thorough understanding of the structure and regulation of the red cell membrane may be a logical stepping stone to unraveling the molecular bases of health problems affecting other cell membranes. We are seeking to elucidate the structure, regulation, biological functions, and pathological states of interactions involving the major membrane protein, termed band 3. Specifically we intend to i) examine the regulation of the association of band 3 with ankyrin (the major linkage between the cytoskeleton and the membrane), ii) characterize the interaction between band 3 and band 4.1, a second major cytoskeletal protein, iii) study the regulation of membrane- cytoskeletal interactions during cell swelling and shrinking, and iv) confirm our mechanisms which explains how the body distinguishes old from young red cells and selectively destroys the former. Research on this letter topic may provide clues for extending the life of stored blood. Research on the former projects should aid in understanding and eventually treating such diseases as sickle cell anemia, hereditary spherocytosis, hereditary elliptocytosis, and hereditary pyropoikilocytosis, as well as maladies of nonerythroid cells. Methods used to investigate the above issues involve predominately protein chemistry, with contributions from immunology and molecular biology.

红细胞膜经常被用作其他生物的模型 膜，因为i)它以高纯度形式大量可用，ii) 它可以在不损害捐赠者的情况下从人类身上获得，iii)其 结构比较简单，iv)主要部件在 几乎所有动物细胞质膜，以及v)病理损害 在其他细胞膜中产生的物质通常表现在红细胞中(例如， 糖尿病、肌肉营养不良等)。因此，一个彻底的 了解红细胞膜的结构和调节可能 成为揭开健康分子基础的合乎逻辑的垫脚石 影响其他细胞膜的问题。我们正在寻求澄清 细胞的结构、调节、生物学功能和病理状态 涉及主要膜蛋白的相互作用，称为带3。 具体地说，我们打算：一)审查对协会的监管 带3的锚蛋白(细胞骨架和细胞周期蛋白的主要连接 膜)，ii)表征带3与带4.1，a之间的相互作用 第二个主要的细胞骨架蛋白，III)研究膜的调节- 细胞膨胀和收缩过程中的细胞骨架相互作用，以及iv) 确认我们的机制，它解释了身体如何区分老与老 年轻的红血球并选择性地破坏前者。关于这方面的研究 字母话题可能为延长储存血液的寿命提供线索。 对以前项目的研究应该有助于理解并最终 治疗镰状细胞性贫血、遗传性球形红细胞增多症、 遗传性椭圆形红细胞增多症和遗传性嗜热红细胞增多症 非红系细胞疾病。用于调查上述问题的方法 问题主要涉及蛋白质化学，贡献来自 免疫学和分子生物学。