RED CELL TYROSINE KINASES AND REGULATION OF METABOLISM

红细胞酪氨酸激酶和代谢调节

• 批准号: 3298950
• 负责人: PHILIP Stewart LOW
• 金额: $ 13.34万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3298950
• 关键词: 6 phosphofructokinase; Mammalia; aldehyde lyase; cell growth regulation; cell transformation; enzyme induction /repression; enzyme inhibitors; enzyme mechanism; enzyme substrate; glycolysis; phosphorylation; protein tyrosine kinase; reticulocytes

With the observation that tyrosine-protein kinases are commonly involved in cell growth control, significant effort has been devoted to understanding the identities, distributions and biochemical actions of these kinases. Such research has recently yielded the curious observation that elevated levels of tyrosine kinase activity are not only associated with rapidly growing and transformed cells, but in some cases also with quiescent, terminally differentiated cells. Since information on the roles of tyrosine kinases in nondividing cells might also provide insight into one or more aspects of their activities in transformed cells, we have undertaken an investigation of the regulation of the tyrosine kinase of erythrocytes and its influence on the properties of the major substrate band 3, especially on its ability to bind and regulate glycolytic enzymes. We intend to purify the above kinase and determine which endogenous/exogenous ligands modulate its activity. We also plan to determine whether activation/inhibition of the kinase in vivo regulates erythrocyte metabolism, as suggested from recent in vitro kinase in vivo regulates erythrocyte metabolism, as suggested from recent in vitro studies. Finally, because glycolytic rates and tyrosine kinase activity are often elevated in transformed cells, we plan to investigate the tyrosine phosphorylation of glycolytic enzyme binding proteins in nonerythroid cells in an attempt to understand the role they may play in the regulation of cell metabolism.

随着酪氨酸蛋白激酶的观察， 在细胞生长控制中，已经进行了大量努力， 了解的身份，分布和生化作用， 这些激酶。这样的研究最近产生了一个奇怪的观察结果 酪氨酸激酶活性水平的升高不仅与 快速生长和转化的细胞，但在某些情况下， 静止的终末分化细胞。由于关于 酪氨酸激酶在非分裂细胞中的作用也可能提供深入了解 转化成一个或多个方面的活动，我们 已经对酪氨酸激酶的调节进行了研究 及其对红细胞主要特性的影响 基质带3，特别是其结合和调节 糖酵解酶我们打算纯化上述激酶并确定 哪些内源性/外源性配体调节其活性。我们还计划 确定体内激酶的激活/抑制是否调节 红细胞代谢，如最近的体外激酶体内研究所示 调节红细胞代谢，如最近的体外研究所示， 问题研究最后，由于糖酵解速率和酪氨酸激酶活性 通常在转化细胞中升高，我们计划研究 糖酵解酶结合蛋白的酪氨酸磷酸化 非红系细胞，试图了解它们可能在 细胞新陈代谢的调节。