STRUCTURE OF PROTEIN-BOUND BILE PIGMENTS

具有共价结合的血红素或线性四吡咯的蛋白质(胆汁 色素)假体基团普遍存在于生物体中。 这些蛋白质在电子传递、光化学和许多方面起作用。 其他角色。这项研究的目的是确定 蛋白质结合体的结构、立体化学和生物合成 胆绿素衍生胆林假体中的四个吡咯 藻胆蛋白群。含四吡咯的小肽 假体基团将以纯形式分离，其结构和 由多种技术组合确定的立体化学，包括1H 核磁共振、快原子轰击质谱仪、圆二色谱、 在可能的情况下，通过与参考化合物进行比较， 利用已知晶体的四聚吡咯蛋白质的降解 结构，或通过合成。一种灵敏的体外荧光分析方法 已经开发出用于测量线性加法的 四吡咯作用于脱藻胆蛋白，并将用于 前体化合物及其合成所涉及的酶的检测 和附着在多肽上。分子遗传学方法在基因工程中应用 这种酶的鉴定也已经设计出来了。

Proteins with covalently bound heme or linear tetrapyrrole (bile pigment) prosthetic groups are present universally in having organisms. These proteins function in electron transport, photochemistry, and many other roles. This research is directed at the determination of the structure, stereochemistry, and biosynthesis of protein-bound tetrapyrroles with specific focus on biliverdin-derived bilin prosthetic groups of the phycobiliproteins. Small peptides bearing tetrapyrrole prosthetic groups will be isolated in pure form and their structure and stereochemistry determined by a combination of techniques, including 1H NMR, fast atom bombardment mass spectrometry, circular dichroism, and, where possible, by comparison with reference compounds, either derived by degradation of tetrapyrrole-bearing proteins with known crystal structures, or by synthesis. A sensitive in vitro fluorescence assay has been developed for the measurement of the addition of linear tetrapyrroles to apophycobiliproteins and will be utilized for the detection of precursor compounds and enzymes involved in their synthesis and attachment to the polypeptides. Molecular genetic approaches to the identification of such enzymes have also been devised.