CHARACTERIZATION OF TRANSCRIPTIONALLY REGULATED GENES

转录调控基因的表征

• 批准号: 3275084
• 负责人: ELIZABETH A CRAIG
• 金额: $ 14.83万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3275084
• 关键词: DNA; Escherichia coli; autoradiography; cytogenetics; endonuclease; environmental stressor; gel electrophoresis; gene expression; genetic manipulation; genetic mapping; genetic strain; genetic transcription; messenger RNA; molecular cloning; monoclonal antibody; nucleic acid denaturation; nucleic acid sequence; radiotracer; structural genes

To gain insight into the mechanisms of gene control, I propose to study the genes related to the major Drosophila heat shock protein of both Drosophila and the bacterium E. coli. The response of organims to heat and other stresses is universal and the proteins induced by treatment have been conserved throughout evolution. We have found a family of genes related to the major heat shcok inducible gene of Drosophila. A number of these genes are transcribed at normal growth temperature and not induced by heat shock. Also we have identified a gene in E. coli which is related to the Drosophila hsp 70 gene and whose transcription is enhanced by heat treatment. The approach to study the regulation and function of these genes will be three-fold. First, we will analyze the transcription of these hsp 70 related genes of Drosophila in different tissues and developmental stages. Secondly, a genetic analysis will be carried out on the 87D Drosophila gene and the E. coli gene to gain insight into the function of these genes. Thirdly, monoclonal antibodies specific for the various protein products will be used to determine the location of these proteins in normal and mutant cells, under normal and heat shock conditions, in order to gain insight into the function of these related proteins. Also, transcription and genetic analysis will be carried out on the 87E actin gene to gain insight into the function and interation of the multigene family of actin genes.

为了深入了解基因控制的机制，我建议研究