GENE ORGANIZATION: STRUCTURE-FUNCTION RELATIONSHIPS

基因组织：结构-功能关系

• 批准号: 3276574
• 负责人: Leslie Anne Leinwand
• 金额: $ 16.43万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-09-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3276574
• 关键词: DNA; Dictyostelium; biological polymorphism; cell growth regulation; cell transformation; chemical structure function; electron microscopy; fungal genetics; gene expression; genetic library; genetic manipulation; genetic markers; genetic transcription; genome; human tissue; hybrid cells; macrophage; molecular cloning; muscle contraction; myoblasts; nucleic acid sequence; structural genes; tissue /cell culture

It is important to understand how the organization of the genome meets the specialized requirements of an organism as complex as the mammal. The goal of this proposal is to study the relationship of DNA sequence organization and function in the mammalian genome. The human myosin heavy chain genes and interspersed repetitive DNA sequences will be studied, particularly as their positions and expression relate to structural gene expression. The myosin heavy chain (MHC) genes code for proteins that are responsible for motility in muscle and nonmuscle systems. The MHC proteins from both systems have very similar function and structure, yet their genes are quite divergent. Nonmuscle MHC genes will be isolated and chromosomally mapped. The organization and sequence of nonmuscle MHC genes will be compared with muscle-specific MHC genes that have been isolated and partially characterized (Leinwand et al. 1983 a & b). A somatic cell genetic approach will be carried out to analyze factors that regulate tissue and developmental-specific expression of muscle and nonmuscle MHC genes. The organization of specific repetitive DNA sequence families in the MHC multigene complex(es) will be determined as well as the muscle-specific expression of any repetitive sequences tested. While analyzing the organization and function of repetitive DNA sequences within the vicinity of the MHC genes, the investigation of the organization and expression of the repetitive sequences scattered throughout the genome will be continued. The interrelationships of repetitive DNA sequences and the muscle and nonmuscle myosin heavy chain genes will provide a better understanding of the link between genomic organization and function.

了解基因组的组织如何满足 像哺乳动物一样复杂的生物体的特殊要求。 目标 这个提案的目的是研究DNA序列组织的关系 和在哺乳动物基因组中的功能。 人类肌球蛋白重链基因 和散布的重复DNA序列将被研究，特别是 它们的位置和表达与结构基因表达有关。 这 肌球蛋白重链 (MHC) 基因编码的蛋白质负责 肌肉和非肌肉系统的运动性。 两者的 MHC 蛋白 系统具有非常相似的功能和结构，但它们的基因却非常相似 发散的。 非肌肉 MHC 基因将被分离并进行染色体定位。 非肌肉 MHC 基因的组织和序列将与 肌肉特异性 MHC 基因已被分离并部分 特征（Leinwand 等人，1983 a & b）。 体细胞遗传 将进行方法来分析调节组织和 肌肉和非肌肉 MHC 基因的发育特异性表达。 这 MHC 中特定重复 DNA 序列家族的组织 将确定多基因复合物以及肌肉特异性 测试的任何重复序列的表达。 在分析时 附近重复 DNA 序列的组织和功能 MHC 基因的组织和表达的研究 分散在整个基因组中的重复序列将是 持续。 DNA重复序列和DNA序列之间的相互关系 肌肉和非肌肉肌球蛋白重链基因将提供更好的 了解基因组组织和功能之间的联系。