A MULTI-FUNCTION IMAGE PROCESSING MICROSCOPE SYSTEM

该项目旨在继续开发、支持和应用 一种多功能视频显微镜系统，包括 最先进的数字控制摄像机、计算机图像 采集、处理和存储，超弱光能力，以及 能够执行空间和时间分辨率的实验 显微生物样本。该系统的设计目标是 荧光和自发光样品的定量测量。在……里面 结合使用生物发光钙的细胞注射技术 指示蛋白Aequorin和各种荧光探针 细胞功能，这个系统正被用来研究各种 刺激/反应效应，包括通过钙介导的刺激/反应效应 离子和/或钙调蛋白；通过光敏色素和 蓝光感受器；脂肪细胞的胰岛素反应；以及 生物发光在细胞水平上的调控。这个 该项目的联合设施正在用于探索生化 刺激中的事件，在完整的活细胞中的反应耦合。特定的 本项目期间的目标是继续开发硬件和 VIDIC显微图像定量分析软件， 改进显微注射和使用化学特异的探针 细胞生物化学，以及对各种 刺激/反应效应。

This project is directed at continued development, support and applications of a multiple function video microscope system which incorporates state-of-the-art digitally controlled video cameras, computer image acquisition, processing and storage, ultra-low light capability, and the ability to perform spatially and temporally resolved experiments on microscopic biological samples. The system has been designed for quantitative measurements of fluorescent and self-luminescent samples. In conjunction with cell injection techniques using the bioluminescent calcium indicator protein, aequorin, and a variety of fluorescent probes of cellular function, this system is being used to investigate a variety of stimulus/response effects including those which are mediated via calcium ions and/or calmodulin; phototropic effects mediated via phytochrome and blue light photoreceptors; insulin responses in adipose cells; and the regulation and control of bioluminescence at the cellular level. The combined facilities of this project are being used to probe the biochemical events in stimulus, response coupling in intact, living cells. Specific aims during this project period are continued developments of hardware and software for quantitative analysis of vidic microscopic images, improvements in microinjection and the use of chemically specific probes of cellular biochemistry, and continued collaborative studies of a variety of stimulus/response effects.