A MULTI-FUNCTION IMAGE PROCESSING MICROSCOPE SYSTEM

多功能图像处理显微镜系统

• 批准号: 3275483
• 负责人: JOHN E WAMPLER
• 金额: $ 7.53万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-02-01 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3275483
• 关键词: adenosine triphosphate; adipocytes; bioluminescence; calcium; egg /ovum; fertilization; fluorescence; image enhancement; insulin; micromanipulator; microscopy; nonvisual photoreceptor; online computer; saltwater environment; single cell analysis; stimulus /response; videotape /videodisc

This project is directed at continued development, support and applications of a multiple function video microscope system which incorporates state-of-the-art digitally controlled video cameras, computer image acquisition, processing and storage, ultra-low light capability, and the ability to perform spatially and temporally resolved experiments on microscopic biological samples. The system has been designed for quantitative measurements of fluorescent and self-luminescent samples. In conjunction with cell injection techniques using the bioluminescent calcium indicator protein, aequorin, and a variety of fluorescent probes of cellular function, this system is being used to investigate a variety of stimulus/response effects including those which are mediated via calcium ions and/or calmodulin; phototropic effects mediated via phytochrome and blue light photoreceptors; insulin responses in adipose cells; and the regulation and control of bioluminescence at the cellular level. The combined facilities of this project are being used to probe the biochemical events in stimulus, response coupling in intact, living cells. Specific aims during this project period are continued developments of hardware and software for quantitative analysis of vidic microscopic images, improvements in microinjection and the use of chemically specific probes of cellular biochemistry, and continued collaborative studies of a variety of stimulus/response effects.

该项目旨在继续开发、支持和应用 多功能视频显微镜系统， 最先进的数字控制摄像机，计算机图像 采集、处理和存储，超低光能力，以及 能够进行空间和时间分辨的实验， 微观生物样本。 该系统的设计目的是 荧光和自发光样品的定量测量。 在 结合使用生物发光钙离子的细胞注射技术 指示蛋白，水母发光蛋白，和各种荧光探针， 细胞功能，该系统被用于研究各种 刺激/反应效应，包括通过钙介导的效应 离子和/或钙调蛋白;通过光敏色素介导的向光效应， 蓝光光感受器;脂肪细胞中的胰岛素反应;以及 在细胞水平上调控生物发光。 的 该项目的组合设施被用于探测生物化学 完整的活细胞中的刺激、反应耦合事件。 具体 本项目期间的目标是继续开发硬件， Vidic显微图像定量分析软件， 显微注射的改进和化学特异性探针的使用 细胞生物化学，并继续合作研究各种 刺激/反应效应。