"21-ICRAD" Nucleic NanoVaccines for Fish

点击翻译按钮获取中文摘要

Aquaculture is the fastest growing food production sector, but it is significantly constrained by infectious diseases. Impact of viral infectionson fish health and welfare is particularly strong, involving important economic losses, and only few vaccines are available. Therefore, it is ofparamount importance to develop new vaccines to prevent fish viral diseases for the development of a sustainable aquaculture.Experimental DNA vaccines against fish viral infections have been developed, some providing high protection but they are injected andcannot be easily administered to young fish. Importantly, several DNA vaccines have been recently made commercially available but remainexpensive. mRNA vaccines hold much hope, but they are just beginning to be tested in fish. In mammals, they often induce strongresponses and good protection. They appear to be fast to produce and safer than DNA vaccines as they should not integrate into hostgenomes.In this context, the NucNanoFish project proposes to establish a nucleic acid platform using biodegradable nanoparticles for efficientdelivery of vaccines, through intra-muscular (IM) or oral/immersion routes, against to well-known viral diseases of severalEuropean farmed fish species. We have selected four different relevant host-pathogen models through cutting edge expertise of eachconsortium's partner. NucNanoFish will design, produce and test DNA/mRNA nucleic acid vaccines loaded or not onto safe-by-designLipoNanoParticles (LNP). LNP are based on a PLA/PLGA (Poly-Lactic/Glycolic Acid) core surrounded with a lipid corona. We hypothesize thattheir efficient uptake by mucosa or the recruitment of Antigen Presenting Cells (APC) at the site of injection will favour a protective immuneICRAD NucNanoFish - ID: 10 3 of 51response at the portal of entry of each relevant virus. To achieve these goals, NucNanoFish has assembled a consortium of six partners, withhighly complementary expertise: one in nanodelivery of antigen and mRNA using LNP, four groups of fish immunovirologists / vaccinologistsand a biotech company in DNA production.