BIOLOGICAL STRUCTURAL STUDIES USING IMAGE PROCESSING
BIOLOGICAL STRUCTURAL STUDIES USING IMAGE PROCESSING
批准号:
3274122
负责人:
PHILLIP ROSS SMITH
金额:
$17.96万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1979
资助国家:
美国
项目状态:
已结题
起止时间:
1979-07-01 至 1987-06-30
中文摘要
我们目前正在研究两种生物结构:晶体肌动蛋白片层
和仙台病毒核衣壳。第一个项目的目的是
确定肌动蛋白的分子结构,分辨率至少为10A。这个
实现这一项目的关键前提是存在
G-肌动蛋白透析法制备高度有序的肌动蛋白薄层晶片
对抗Gd离子。具体目标是:获取三维模型
肌动蛋白的分辨率为15A,由倾斜的电子显微镜确定
晶体肌动蛋白片材的负染制剂的视图,以及
通过以下方法找出肌动蛋白分子在肌动蛋白细丝中的方向
通过计算将从肌动蛋白片材获得的模型与模型对齐
来源于肌动蛋白细丝的副晶体。
包膜病毒的核衣壳是两种重要的结构
作为研究体内蛋白质合成和运输的模型系统
这些细胞本身就是致病病毒的重要组成部分。这个
我们项目的目的是研究仙台病毒的结构
核衣壳。我们的目标是确定蛋白质在体内的几何堆积
核衣壳和单链RNA的位置。
英文摘要
We are currently studying two biological structures: crystalline actin sheets
and the Sendai virus nucleocapsid. The purpose of the first project is to
determine the molecular structure of actin to at least 10 A resolution. The
crucial prerequisite for the realization of this project is the existence of
highly ordered thin crystalline sheets of actin prepared by dialysis of G-actin
against gadolinium ions. The specific aims are: To obtain 3-dimensional model
of actin to 15 A resolution, determined from electron micrographs from tilted
views of negatively stained preparations of crystalline actin sheets, and to
find the orientation of the actin molecule within the actin filament by
computationally aligning the model obtained from actin sheets with the model
derived from paracrystals of actin filaments.
The nucleocapsids of enveloped viruses are structures of major importance both
as model-systems for the study of the synthesis and transport of proteins within
cells, and in their own right as vital components of pathogenic viruses. The
purpose of our project is to study the structure of the Sendai virus
nucleocapsid. We aim to determine the geometrical packing of protein within the
nucleocapsid and the location of the single RNA strand.
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CORE--COORDINATED COMPUTER FACILITY
-
批准号:6268908
-
项目类别:
-
资助金额:$18.77万
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财政年份:1997
-
负责人:PHILLIP ROSS SMITH
-
依托单位:
CORE--COORDINATED COMPUTER FACILITY
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批准号:6236313
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项目类别:
-
资助金额:$19.05万
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财政年份:1996
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负责人:PHILLIP ROSS SMITH
-
依托单位:
CORE--COORDINATED COMPUTER FACILITY
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批准号:3729082
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:PHILLIP ROSS SMITH
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依托单位:
CORE--COORDINATED COMPUTER FACILITY
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批准号:5206814
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:PHILLIP ROSS SMITH
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依托单位:--
CORE--COORDINATED COMPUTER FACILITY
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批准号:3749154
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:PHILLIP ROSS SMITH
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依托单位:
海外基金