SMALL NUCLEAR RNA SYNTHESIS IN DEVELOPMENT

正在开发的小核 RNA 合成

• 批准号: 3275037
• 负责人: WILLIAM F. MARZLUFF
• 金额: $ 14.35万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-05-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3275037
• 关键词: Saccharomyces; animal population genetics; cytogenetics; developmental genetics; gene expression; genetic manipulation; genetic mapping; genetic transcription; growth /development; human tissue; laboratory mouse; mammalian embryology; molecular cloning; nonmammalian vertebrate embryology; nucleic acid hybridization; nucleic acid metabolism; nucleic acid sequence; nucleoproteins; saltwater environment; sea urchins; small nuclear RNA

The objective of the project is to understand how gene activity is controlled in early development in sea urchin embryos and in mouse embryos. Using cloned genes for snRNAs we will determine the sequences required for correct developmental regulation of gene expression. We will use an in vivo system, microinjection of DNA into sea urchin embryos, and an in vitro s- dependent system which we have developed. Protein factors involved in transcription and 3' end formation of the sea urchin U1 RNA will be characterized and we will attempt to detect factors involved in developmental regulation. We will also determine the sequence requirements for correct tissue-specific expression of the mouse U1b genes. Additional sea urchin snRNA genes will be isolated and characterized.

该项目的目标是了解基因活性如何 受控于海胆胚胎的早期发育和 小鼠胚胎。 使用 snRNA 的克隆基因，我们将 确定正确发育所需的序列 基因表达的调控。 我们将使用体内系统， 将 DNA 显微注射到海胆胚胎中，并进行体外实验 我们开发的依赖系统。 蛋白质因子 参与海胆 U1 的转录和 3' 末端形成 RNA 将被表征，我们将尝试检测因素 参与发育调节。 我们还将确定 正确的组织特异性表达的序列要求 小鼠 U1b 基因。 额外的海胆 snRNA 基因将 分离并表征。