SMALL NUCLEAR RNA SYNTHESIS IN DEVELOPMENT

该项目的目标是了解基因活动是如何 控制在海胆胚胎的早期发育和在 小鼠胚胎。将克隆的基因用于SnRNA，我们将 确定正确发育所需的序列 基因表达的调控。我们将使用体内系统， 将DNA显微注射到海胆胚胎中，并建立了一种体外S- 我们开发的依赖系统。蛋白质因子 参与海胆U1的转录和3‘端的形成 将对RNA进行表征，我们将尝试检测各种因素 参与发育调节的。我们还将确定 正确的组织特异性表达的序列要求 小鼠的U1b基因。额外的海胆SnRNA基因将是 与世隔绝的，有特点的。

The objective of the project is to understand how gene activity is controlled in early development in sea urchin embryos and in mouse embryos. Using cloned genes for snRNAs we will determine the sequences required for correct developmental regulation of gene expression. We will use an in vivo system, microinjection of DNA into sea urchin embryos, and an in vitro s- dependent system which we have developed. Protein factors involved in transcription and 3' end formation of the sea urchin U1 RNA will be characterized and we will attempt to detect factors involved in developmental regulation. We will also determine the sequence requirements for correct tissue-specific expression of the mouse U1b genes. Additional sea urchin snRNA genes will be isolated and characterized.