Next Generation Sequencing and Genotyping Core Facility

下一代测序和基因分型核心设施

• 批准号: 8340327
• 负责人: WILLIAM F. MARZLUFF
• 金额: $ 27.48万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-23 至 2015-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8340327
• 关键词: Address; Adopted; Advisory Committees; Animal Model; Archives; Area; Award; Bioinformatics; Biomedical Research; Budgets; Cancer Center; Cancer Center Support Grant; Cancer Hospital; Capillary Electrophoresis; Cells; Chemistry; Chromatin; Chromatin Structure; Commit; Complex; Copy Number Polymorphism; Core Facility; DNA; DNA Methylation; DNA Resequencing; DNA Sequence; Data; Data Analyses; Data Quality; Detection; Development; Ensure; Equipment; Evaluation; Exons; Faculty; Fees; Funding; Future; Gene Expression; Genes; Genetic Polymorphism; Genetic Transcription; Genome; Genomics; Genotype; Goals; Grant; Growth; Hour; Housing; Human; Human Resources; Individual; Information Technology; Institutes; Institution; Investments; Laboratories; Malignant Neoplasms; Measures; MicroRNAs; Microsatellite Repeats; Normal Cell; North Carolina; Paper; Patients; Peer Review; Positioning Attribute; Preparation; Price; RNA; RNA Sequences; RNA analysis; Reading; Research; Research Personnel; Resources; SNP genotyping; Sampling; Scientist; Services; Site; Solid; Technology; The Cancer Genome Atlas; Time; Training; Universities; Variant; Wages; Whole-Genome Shotgun Sequencing; Work; anticancer research; base; cost; cost effective; digital; experience; genome sequencing; genome wide association study; genome-wide; genotyping technology; high throughput technology; human tissue; mRNA Expression; medical schools; member; neoplastic cell; new technology; next generation; rapid growth; single molecule; success; technology development; trait; tumor

Next Generation Sequencing and Genotyping Core Facility. The goal of the Next Gen Sequencing component is to make "next generation" sequencing and related services widely available to UNC LCCC members at cost-effective prices. Services include: whole genome shotgun sequencing of human and model organisms, chromatin-IP sample sequencing, measuring RNA expression with both digital gene expression and RNA-seq, SNP typing, and polymorphism screens. The component adds value to the cancer center by putting complex and expensive DNA/RNA sequencing technology within easy reach of UNC LCCC members. Strongly integrated genomics and bioinformatics groups interact with the user to ensure robust data that is archived appropriately for future use. This component is key in UNC LCCC's participation in The Cancer Genome Atlas project and serving LCCC needs. Future plans Include expanding staff and equipment so that a full array of Next Gen sequencing and services may be provided in a timely manner, as well as the acquisition of the next generation of technology. The goal of the DNA Sequencing component is to produce high quality sequencing and genotyping data rapidly and at a reasonable cost for UNC-CH researchers using state-of-the-art technologies as well as provide technical support to enhance the value of results produced. Services include DNA sequencing and microsatellite genotyping. The component adds value to the cancer center by producing high-quality data at a competitive price while providing the benefits of a local resource to assist in: scientific strategies, sample preparation, sequencing through difficult regions, and assistance in data interpretation. This component has a wide user base with over 220 different laboratories as users in 2009. Future plans include continued use of existing equipment, reduction of chemistry costs, and evaluation of future sequencing technology options such as long read single molecule sequencing technologies. The goal of the High Throughput Genotyping component is to produce high-quality genotyping data rapidly and at a reasonable cost for researchers using state-of-the-art technologies. Services include SNP genotyping, copy number variation and DNA methylation profiling analysis. New technologies and/or applications are being explored to increase the core's competitive edge and to allow for Genome Wide Association Study on rare variants. These core components are used by multiple members and in each core, peer reviewed member used exceeds 70%. The proposed budget of $241,797 is less than 5% of the core's operating cost. The budget provides salary stability for vital core staff who work with members to adopt these novel technologies.

下一代测序和基因分型核心设施。 下一代测序组件的目标是以具有成本效益的价格向 UNC LCCC 成员广泛提供“下一代”测序和相关服务。服务包括：人类和模式生物的全基因组鸟枪测序、染色质 IP 样本测序、通过数字基因表达和 RNA-seq 测量 RNA 表达、SNP 分型和多态性筛选。这 该组件通过将复杂且昂贵的 DNA/RNA 测序技术置于 UNC LCCC 成员的手中，为癌症中心增添了价值。高度集成的基因组学和生物信息学小组与用户互动，以确保正确存档可靠的数据以供将来使用。该组件对于 UNC LCCC 参与癌症基因组图谱项目和满足 LCCC 需求至关重要。未来计划包括扩大人员和设备，以便及时提供全套下一代测序和服务，以及收购下一代技术。 DNA 测序组件的目标是使用最先进的技术以合理的成本为 UNC-CH 研究人员快速生成高质量的测序和基因分型数据，并提供技术支持以提高所产生结果的价值。服务包括 DNA 测序和微卫星基因分型。该组件通过在以下位置生成高质量数据为癌症中心增加价值： 具有竞争力的价格，同时提供当地资源的优势，以协助：科学策略、样品制备、困难区域测序以及数据解释协助。该组件拥有广泛的用户基础，2009 年有超过 220 个不同的实验室作为用户。未来的计划包括继续使用现有设备、降低化学成本以及评估未来测序技术选项 例如长读长单分子测序技术。 高通量基因分型组件的目标是使用最先进的技术以合理的成本为研究人员快速生成高质量的基因分型数据。服务包括 SNP 基因分型、拷贝数变异和 DNA 甲基化谱分析。正在探索新技术和/或应用，以提高核心的竞争优势，并允许对罕见变异进行全基因组关联研究。 这些核心组件被多个成员使用，并且在每个核心中，同行评审成员使用的比例超过 70%。拟议预算为 241,797 美元，不到核心运营成本的 5%。该预算为与会员合作采用这些新技术的重要核心员工提供了薪资稳定性。