CHARACTERIZATION OF TRANSCRIPTIONALLY REGULATED GENES

为了深入了解基因控制的机制，我建议研究 与果蝇主要热休克蛋白相关的基因 和细菌E.杆菌 有机体对热的反应和其他 应激是普遍的，由处理诱导的蛋白质已经被 在进化过程中保持不变。 我们已经发现了一个基因家族 果蝇的主要热休克诱导基因。 其中一些基因 在正常生长温度下转录，不受热诱导 冲击. 我们还在E.大肠杆菌， 果蝇热休克蛋白70基因及其转录被热增强 治疗 研究这些调节和功能的方法 基因将是三倍。 首先，我们将分析 这些果蝇不同组织中的hsp 70相关基因， 发育阶段 其次，将对以下方面进行遗传分析： 果蝇87D基因和E.大肠杆菌基因来深入了解 这些基因的功能。 第三，特异性针对 将使用各种蛋白质产品来确定这些蛋白质的位置。 蛋白质在正常和突变细胞，在正常和热休克 条件，以便深入了解这些相关的功能 proteins. 此外，转录和遗传分析将在 87E肌动蛋白基因，以深入了解功能和相互作用， 肌动蛋白基因的多基因家族

To gain insight into the mechanisms of gene control, I propose to study the genes related to the major Drosophila heat shock protein of both Drosophila and the bacterium E. coli. The response of organims to heat and other stresses is universal and the proteins induced by treatment have been conserved throughout evolution. We have found a family of genes related to the major heat shcok inducible gene of Drosophila. A number of these genes are transcribed at normal growth temperature and not induced by heat shock. Also we have identified a gene in E. coli which is related to the Drosophila hsp 70 gene and whose transcription is enhanced by heat treatment. The approach to study the regulation and function of these genes will be three-fold. First, we will analyze the transcription of these hsp 70 related genes of Drosophila in different tissues and developmental stages. Secondly, a genetic analysis will be carried out on the 87D Drosophila gene and the E. coli gene to gain insight into the function of these genes. Thirdly, monoclonal antibodies specific for the various protein products will be used to determine the location of these proteins in normal and mutant cells, under normal and heat shock conditions, in order to gain insight into the function of these related proteins. Also, transcription and genetic analysis will be carried out on the 87E actin gene to gain insight into the function and interation of the multigene family of actin genes.