CHARACTERIZATION OF TRANSCRIPTIONALLY REGULATED GENES

为了深入了解基因控制的机制，我建议研究 两种果蝇主要热休克蛋白的相关基因 和细菌E.Coli。有机胺对热和其他物质的反应 压力是普遍存在的，治疗诱导的蛋白质已经 在整个进化过程中都是保守的。我们已经发现了一组与 果蝇的主要热休克诱导基因。这些基因中的许多 是在正常生长温度下转录的，不受热诱导 令人震惊。此外，我们还在大肠杆菌中发现了一个与 果蝇HSP70基因及其在高温下转录增强的研究 治疗。研究它们的调节和作用的途径 基因将是三重的。首先，我们来分析一下 果蝇HSP70相关基因在不同组织和组织中的表达 发育阶段。其次，将对 87D果蝇基因和大肠杆菌基因，以深入了解 这些基因的功能。第三，针对人类免疫缺陷病毒的单抗 不同的蛋白质产品将被用来确定这些 正常和热休克条件下正常细胞和突变细胞中的蛋白质 条件，以深入了解这些相关的功能 蛋白质。此外，还将进行转录和遗传分析 87E肌动蛋白基因以深入了解肌动蛋白的功能和相互作用 肌动蛋白基因的多基因家族。

To gain insight into the mechanisms of gene control, I propose to study the genes related to the major Drosophila heat shock protein of both Drosophila and the bacterium E. coli. The response of organims to heat and other stresses is universal and the proteins induced by treatment have been conserved throughout evolution. We have found a family of genes related to the major heat shcok inducible gene of Drosophila. A number of these genes are transcribed at normal growth temperature and not induced by heat shock. Also we have identified a gene in E. coli which is related to the Drosophila hsp 70 gene and whose transcription is enhanced by heat treatment. The approach to study the regulation and function of these genes will be three-fold. First, we will analyze the transcription of these hsp 70 related genes of Drosophila in different tissues and developmental stages. Secondly, a genetic analysis will be carried out on the 87D Drosophila gene and the E. coli gene to gain insight into the function of these genes. Thirdly, monoclonal antibodies specific for the various protein products will be used to determine the location of these proteins in normal and mutant cells, under normal and heat shock conditions, in order to gain insight into the function of these related proteins. Also, transcription and genetic analysis will be carried out on the 87E actin gene to gain insight into the function and interation of the multigene family of actin genes.