Structural studies of the Apicomplexan glideosome-associated connector platform

寄生虫在不同的生物体中生长，虽然它通常对宿主没有好处，但它经常会引起疾病。顶复合体寄生虫是一种单细胞的类动物生物，可引起人类和动物的一系列疾病。值得注意的是，恶性疟原虫是致死性疟疾的主要病因，而。刚地弓形虫是世界上最常见的寄生虫之一，具有重要的兽医和医学意义，因为它可以导致流产或先天性疾病。它感染人类、野生动物和家畜，包括鸟、猫、绵羊、山羊、牛、猪和家禽。弓形虫病在全球范围内具有巨大的社会经济和健康影响。这些寄生虫生存的核心是它们独特的移动能力，这使得它们能够瞄准并入侵宿主细胞，在宿主细胞内生活，然后逃跑。这些寄生虫进入和逃离目标细胞的分子策略是共享的。一组蛋白质在寄生虫体内形成了一个分子机器，被称为滑翔体。最近发现了一种新的蛋白，即滑翔体相关连接蛋白（glideosomes -associated connector， GAC），它代表了宿主细胞和寄生虫细胞骨架之间的重要分子联系。这个立足点允许寄生虫抓住宿主细胞并移动。本研究项目旨在研究滑翔体相关连接蛋白（GAC）的分子形状和相互作用，并定义这个多组分系统的结构。这一信息将帮助我们在原子水平上了解这些寄生虫是如何移动的，而且由于这一过程对寄生虫的生存至关重要，它将为合理设计新的疫苗策略提供新的指导。

A parasite grows in a different organism and while it is usually not a benefit to the host, it can often cause disease. The Apicomplexan parasites are single-celled, animal-like organisms which cause a range of diseases in humans and animals. Notably, Plasmodium falciparum is the chief cause of fatal malaria, while. Toxoplasma gondii, one of the world's most common parasites, and is of veterinary and medical importance, as it can cause abortion or congenital disease. It infects humans, wild and domestic animals, including birds, cats, sheep, goats, cattle, pigs and poultry. Toxoplasmosis has an enormous socio-economic and health impacts across the globe. Central to the survival of these parasite is their distinct ability to move (be motile), which allows them to target and invade host cells, live inside and subsequent escape. The molecular strategies that these parasite species employ to enter and escape their target cells are shared. An assembly of proteins form a molecular machine inside the parasite, known as the glideosome. A new protein, the glideosome-associated connector (GAC) has been recently discovered which represents a crucial molecular link between the host cell and the parasite cellular skeleton. This foothold allows the parasite to grip and move with respect to the host cell.This research project aims investigate the molecular shapes and interactions of the glideosome-associated connector (GAC) protein and define the architecture of this multi-component system. This information will help us to see at the atomic level how these parasites can move, and as this process is essential for parasite survival it will provide new guidance for the rational design of new vaccine strategies.