ION MOBILITY SPECTROMETRY

生物体液等复杂混合物的气相分析 层析通常需要使用选择性检测器来 歧视干扰物种和/或加强对 想要的化合物。然而，除了质谱仪外，大多数 GC检测器不是很难就是不可能在很宽的范围内工作 多种选择性和非选择性模式。尽管不是设计成 提供质谱仪提供的数据范围和范围，离子 迁移率光谱仪具有定量和低分辨率的特点 有机化合物的定性检测。与一般情况相比 采用了选择性探测器，如电子捕获和 氮磷探测器，离子迁移率光谱仪提供 具有可调选择性的明显优势。因为它的多功能性和 与质谱计相比，该仪器成本低，具有 在生物样品中对许多药物进行常规筛选的潜力很大。 在过去的资金支持期间，我们扩大了ion的能力 基于傅里叶变换模式的迁移率光谱分析 行动的关键。傅里叶变换离子迁移率光谱联用 在本申请中提出的超临界流体色谱将 为定量和定量分析提供强大的分析工具 微量生物重要物质的定性测定 挥发性不足以被气体洗脱的化合物 层析，但包含在太复杂的混合物中，无法分离 液体色谱学。

The analysis of complex mixtures such as biological fluids by gas chromatography often requires the use of selective detectors to discriminate against interfering species and/or enhance the response to desired compounds. However, with the exception of mass spectrometers, most GC detectors are either difficult or impossible to operate in a wide variety of selective and nonselective modes. Although not designed to provide the range and scope of data provided by mass spectrometers, the ion mobility spectrometer is capable of quantitative and low resolution qualitative detection of organic compounds. When compared with commonly employed selective detectors such as the electron capture and nitrogen-phosphorus detectors, the ion mobility spectrometer offers the distinct advantage of tunable selectivity. Because of its versatility and low cost when compared to that of mass spectrometers, the instrument has great potential for routine screening of many drugs in biological samples. During the past funding period we have expanded the capabilities of ion mobility spectrometry through the development of a Fourier transform mode of operation. Coupling Fourier transform ion mobility spectrometry with supercritical fluid chromatography as is proposed in this application will produce a powerful analytical instrument for both quantitative and qualitative determination of trace quantities of biologically important compounds that are not sufficiently volatile to be eluted by gas chromatography but are contained in mixtures too complex to be separated by liquid chromatography.