A Novel Biosensor Based on Liquid Phase Ion Mobility

描述(由申请人提供)： 该提案要求探索和开发资金，以便为能够对复杂生物混合物进行超高分辨率化学分离的新型生物分析微型传感器产生概念验证数据。到目前为止，所有已经开发的生物传感器本质上都是一维的。也就是说，每个传感器被设计为仅监控一种或一类化学品。基于高分辨率分离技术的多维微传感器将允许同时监测多个目标分析物。尽管过去已经尝试了各种小型化的分离方法，如层析、电泳和离子迁移率光谱，但为了小型化，分辨率总是被牺牲掉。目前还没有小型分离设备可以达到与桌面分离设备类似的分辨率。在这个项目中，我们建议开发一种高分辨率的分离传感器，它需要一个微型设备，并且有可能获得比目前传统分离方法更高的分辨率。这种设备的开发将真正成为分离科学的重大突破，并显著提高监测健康的分析能力。

DESCRIPTION (provided by applicant): This proposal requests exploratory and developmental funds to generate proof of concept data for a novel bio-analytical micro-sensor capable of ultra high-resolution chemical separations of complex bio-mixtures. To date, all bio-sensors that have been developed are mono-dimensional in nature. That is, each sensor is designed to monitor only one chemical or class of chemicals. A multi-dimensional micro-sensor, based on high-resolution separation technology, would permit simultaneous monitoring of multiple target analytes. Although various attempts to miniaturize separation methods such as chromatography, electrophoresis and ion mobility spectrometry have been attempted in the past, resolving power is always sacrificed for miniaturization. There are no examples of diminutive separation devices that achieve a resolving power similar to that of their desk top counterparts. For this project, we propose to develop a high-resolution separation sensor that requires a miniature device and is potentially capable of achieving resolving powers greater than those currently possible by traditional separation methods. The development of such a device would truly be a major breakthrough in separation science and significantly improve analytical capabilities for monitoring health.