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Using systems biology to determine how budding yeast coordinates carbon and nitrogen sensing for efficient growth

利用系统生物学确定出芽酵母如何协调碳和氮传感以实现高效生长

基本信息

批准号：
BB/W006545/1
负责人：
Peter Swain
金额：
$ 73.85万
依托单位：
University of Edinburgh
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2022
资助国家：
英国
起止时间：
2022 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FW006545%2F1
关键词：
Using systems biology determine how

项目摘要

A hallmark of life is the ability to sense and respond to change, and even unicellular organisms have this ability. They are able to sense changes in the availability of nutrients and adjust the rate at which they grow to new environments. Although we know the proteins involved in making this choice, we do not understand how they interact to enable cells to "decide" appropriately. A difficulty is monitoring the proteins as they perform decision-making. We will use bakers' yeast, a cell with a nucleus like our own cells, where we can measure its decision-making activity by the shuttling of proteins in and out of the nucleus. By exposing cells to changing levels of carbon and nitrogen sources - two essential nutrients, and by transiently removing proteins that are key to signalling, we will characterise how sensing is coordinated with growth. Through integrating this data with mathematical modelling, we will infer the decision-making strategy that is most supported by our results. Evolution means that yeast cells are related to human cells, and signalling proteins in yeast are similar to our own signalling proteins. This task of coordinating growth with the availability nutrients is fundamental, and the strategy we uncover should therefore be general and operate too in other organisms.

生命的一个标志是感知变化并对变化做出反应的能力，即使是单细胞生物也具有这种能力。它们能够感知营养供应的变化，并调整它们适应新环境的速度。尽管我们知道参与做出这一选择的蛋白质，但我们不知道它们如何相互作用，使细胞能够做出适当的决定。一个困难是在蛋白质执行决策时对其进行监控。我们将使用面包师酵母，这是一种像我们自己的细胞一样有核的细胞，我们可以通过将蛋白质往返于细胞核内来测量其决策活动。通过将细胞暴露在碳和氮源--两种基本营养物质--水平的变化中，以及通过瞬时移除信号关键的蛋白质，我们将描述感知与生长是如何协调的。通过将这些数据与数学模型相结合，我们将推断出最受我们结果支持的决策策略。进化意味着酵母细胞与人类细胞有亲缘关系，酵母中的信号蛋白与我们自己的信号蛋白相似。这种协调生长和可获得营养的任务是基本的，因此我们发现的策略应该是通用的，也应该在其他生物体中运作。