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Understanding the regulation of glucose sensing and transport in budding yeast using dynamic inputs

使用动态输入了解芽殖酵母中葡萄糖传感和运输的调节

基本信息

批准号：
BB/R001359/1
负责人：
Peter Swain
金额：
$ 61.05万
依托单位：
University of Edinburgh
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR001359%2F1
关键词：
Understanding regulation glucose sensing transport

项目摘要

Glucose is the preferred source of energy for nearly all cells, from microbes to plants to animals, but we do not understand how cells sense and respond to changes in glucose's availability. We propose to study the sensing and uptake of glucose in budding yeast, one of the simplest organisms that has a similar structure to our own cells. Yeast has seven main transporters for glucose, and our focus is to understand why so many are necessary and how only the appropriate transporters are present at the appropriate time. To do so, we will study both populations of and individual cells and probe their behaviour in fluctuating levels of glucose to determine if particular transporters increase in number in response to either the concentration of extracellular glucose or to how that extracellular concentration changes with time. We will thus determine under which conditions each transporter is optimum and has its highest levels. Further, using mutations to disrupt sensing and mathematical modelling of our results, we will discover how the underlying biochemistry works to ensure a suitable rate of uptake of glucose through changing the types of transporters present. Our results will both address the fundamental question of how cells can regulate their import of glucose and provide quantitative measurements of the levels of the transporters that can be exploited by the biotechnology industry to, for example, increase production of valuable biological compounds.

葡萄糖是几乎所有细胞的首选能量来源，从微生物到植物再到动物，但我们不了解细胞如何感知和响应葡萄糖可用性的变化。我们建议研究葡萄糖在芽殖酵母中的感知和摄取，芽殖酵母是最简单的生物体之一，与我们自己的细胞具有相似的结构。酵母有七种主要的葡萄糖转运蛋白，我们的重点是了解为什么这么多是必要的，以及如何只有适当的转运蛋白在适当的时间存在。要做到这一点，我们将研究群体和单个细胞，并探测它们在葡萄糖水平波动中的行为，以确定特定转运蛋白的数量是否增加，以响应细胞外葡萄糖的浓度或细胞外浓度如何随时间变化。因此，我们将确定在哪些条件下每个运输商是最佳的，并有其最高水平。此外，使用突变来破坏我们结果的传感和数学建模，我们将发现基础生物化学如何通过改变存在的转运蛋白的类型来确保葡萄糖摄取的适当速率。我们的研究结果将解决细胞如何调节葡萄糖输入的基本问题，并提供可被生物技术行业利用的转运蛋白水平的定量测量，例如，增加有价值的生物化合物的产量。