GENETIC ANALYSIS OF ENZYME PROCESSING AND LOCALIZATION

酶加工和定位的遗传分析

• 批准号: 3277345
• 负责人: ELIZABETH W JONES
• 金额: $ 17.13万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-07-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3277345
• 关键词: Saccharomyces; antibody formation; carboxypeptidase; gel electrophoresis; gene complementation; gene expression; gene mutation; genetic mapping; molecular cloning; plasmids; vesicle /vacuole

The long term goal of this program is to delineate the pathway by which the vacuolar hydrolases of the primitive eukaryote Saccharomyces cerevisiae are processed and localized to the vacuole. Our specific aims are to isolate and characterize mutations which affect several vacuolar hydrolase activities simultaneously and to gain an understanding of the way in which the pleiotropic effect is achieved. By examination of the properties of doubly mutant strains in which one of the two mutations has been characterized as to the level or location within the pathway of the blockage, the order of mutations within the pathway can be determined. A second aim is to clone an study the genes and gene prodducts inactivated by the mutations, and to develop immunochemical reagents to allow monitoring of the levels and intracellular location of these gene products. A longer term goal is to understand what the function of these gene products is in the pathway.

这一计划的长期目标是勾勒出 原始真核生物酿酒酵母的液泡水解酶是 加工并定位到液泡上。我们的具体目标是孤立 并表征了影响几种液泡水解酶的突变 同时进行各种活动，并了解 实现了多效性效应。通过对属性的检查 两个突变中的一个已经发生突变的双突变菌株 在路径中的水平或位置的特征的 阻断后，可以确定该途径内突变的顺序。一个 第二个目标是克隆一项研究，这些基因和基因产物被 突变，并开发免疫化学试剂以允许监测 这些基因产物的水平和细胞内位置。一个更长的 学期目标是了解这些基因产物的功能。 这条小路。