GENETIC ANALYSIS OF ENZYME PROCESSING AND LOCALIZATION

该计划的长期目标是描绘出 原始真核生物酿酒酵母的液泡水解酶是 加工并定位于液泡。 我们的具体目标是 并表征影响几种液泡水解酶的突变 同时进行活动，并了解如何 实现了多效性效果。 通过检查的性质 双突变菌株，其中两个突变之一已经 其特征在于在所述路径内的水平或位置， 通过阻断，可以确定途径内的突变顺序。 一 第二个目的是克隆研究基因和基因产物， 突变，并开发免疫化学试剂， 这些基因产物的水平和细胞内位置。 更长 长期目标是了解这些基因产物的功能是什么， 这条路

The long term goal of this program is to delineate the pathway by which the vacuolar hydrolases of the primitive eukaryote Saccharomyces cerevisiae are processed and localized to the vacuole. Our specific aims are to isolate and characterize mutations which affect several vacuolar hydrolase activities simultaneously and to gain an understanding of the way in which the pleiotropic effect is achieved. By examination of the properties of doubly mutant strains in which one of the two mutations has been characterized as to the level or location within the pathway of the blockage, the order of mutations within the pathway can be determined. A second aim is to clone an study the genes and gene prodducts inactivated by the mutations, and to develop immunochemical reagents to allow monitoring of the levels and intracellular location of these gene products. A longer term goal is to understand what the function of these gene products is in the pathway.