STRUCTURE AND REPLICATION OF POTYVIRUSES

本提案涉及《公约》的结构、翻译和复制， 马铃薯Y病毒以烟草蚀纹病毒为模型系统。 马铃薯Y病毒 是影响几种重要食物的最大的植物病毒群， 纤维和园艺作物。 本提案的目的是研究 这些病毒的分子生物学，因为我们相信， 获得的信息将增强我们对基本原则的理解 潜在的病毒基因组组织和复制。 建议： (1)通过测序、克隆和翻译来表征TEV的基因组 （2）确定一些相似性或差异之间的研究 不同的马铃薯Y病毒的基因组;（3）研究的复制 通过分析植物中病毒相关的亚基因组RNA和存在 病毒相关蛋白质;（4）确定翻译的性质 （5）确定亚基因组RNA种类的性质; 病毒相关包涵体蛋白。 Potyviruses与 动物小RNA病毒如脊髓灰质炎具有聚（A）尾， 基因组相关蛋白 然而，两者之间存在着重要的区别。 因此 例如，脊髓灰质炎和其他相关病毒有一个翻译策略， 包括产生单个大多肽，然后 已处理。 另一方面，马铃薯Y病毒似乎有一个不同的 涉及亚基因组RNA物种的遗传的策略。 的确切 这些亚基因组物种的起源及其功能尚不清楚。 此外，多病毒复制策略涉及产生 特定类型的包涵体。 因此，虽然马铃薯Y病毒属是 在结构上有点类似于动物小RNA病毒， 基因组，它们的复制和翻译策略似乎 不同. 有鉴于此，对这些病毒的研究 将引导我们对病毒的基本生物学产生一些新的概念， 将军

This proposal deals with the structure, translation and replication of potyviruses using tobacco etch virus as the model system. The potyviruses are the largest group of plant viruses affecting several important food, fiber and horticultural crops. The objective of this propsal is to study the molecular biology of these viruses, since we believe that the information gained would enhance our understanding of the basic principles underlying virus genome organization and replication. It is proposed to: (1) characterize the genome of TEV by sequencing, cloning and translational studies; (2) determine some of the similarities or disparities between the genome of different available potyviruses; (3) study the replication of the virus in the plant by analysis of virus-related subgenomic RNA and presence of virus-related proteins; (4) determine the nature of the translational products of the subgenomic RNA species; (5) characterize the nature of the virus-related inclusion body proteins. The p]otyviruses are similar to the animal picarnaviruses such as polio in having a poly(A) tail and genome-linked protein. However, there are important differences. Thus, for example, polio and other related viruses have a translational strategy involving the production of a single large polypeptide which is then processed. The potyviruses on the other hand, appear to have a different strategy involving the geneation of sub-genomic RNA species. The exact origin of these sub-genomic species and their function is not known. Further, the polyvirus replication strategy involves the production of specific types of inclusion bodies. Thus, although the potyviruses are somewhat structurally similar to the animal picarnaviruses with respect to the genome, their replicaton and translational strategies appear to be different. In view of these it is likely that a study of these viruses would lead us to some new concepts about the basic biology of viruses in general.