GENETIC ANALYSIS OF BACTERIAL PHOTOSYNTHESIS
GENETIC ANALYSIS OF BACTERIAL PHOTOSYNTHESIS
批准号:
3279880
负责人:
SAMUEL KAPLAN
金额:
$21.98万
依托单位国家:
美国
项目类别:
财政年份:
1989
资助国家:
美国
项目状态:
已结题
起止时间:
1989-08-01 至 1991-07-31
关键词:
Escherichia coli Rhodopseudomonas bacterial DNA bacterial genetics electron transport gene expression genetic manipulation genetic regulation laboratory rabbit membrane activity membrane structure microorganism culture molecular cloning mutant nucleic acid probes nucleic acid sequence operon photosynthesis photosynthetic bacteria plasmids pseudogenes structural genes
中文摘要
利用兼性光营养细菌球形红假单胞菌,
我们正试图在分子水平上理解基因控制
诱导光合膜系统(ICM)。人类的遗传基础
ICM的表达延伸到:1.主要的细菌叶绿素蛋白
光谱复合体、细胞色素、ATPase和其他ICM相关酶
活性和结构蛋白,2.类胡萝卜素和
细菌-叶绿素合成,3.磷脂生物合成酶和
磷脂的运动,以及4.导致
细胞对氧分压和光照变化的适应
强度,以及那些有序地参与组装的机构,
以及ICM的结构-功能相互关系。它是
建议我们识别、克隆和表征这些基因区域
与ICM结构和法规相关。这项研究包括,结构
通过限制性内切酶分析,DNA区域的
基因表达的测序和体外和体内表征。
我们建议制定程序,以选择、隔离和
在体内和体外,突变的特征是
与ICM结构和功能相关的应用
脱氧寡核苷酸和转座子诱导的突变。我们打算
衍生遗传系统以监测这些克隆区域在
同源和异源寄主,以便更好地了解
基因控制的机制。这样的研究将直接影响到更广泛的
关于调节信号的性质及其在
异种寄主系统。最后,我们打算开发基因和
运动、互补、表达和表达的伪遗传系统
球状乳杆菌遗传区的简易处理。我们将尝试
发展接合和转导系统、粘粒、质粒、启动子
使用经典原核基因技术的克隆工具等
以及重组DNA技术。了解功能和
光合作用的结构含义是充分利用
营养,因此,人类人口的健康。平等的
重要性,结构与功能的相互关系
膜相关活动对健康至关重要,因为
与健康有关的膜现象的性质,例如膜受体,
线粒体功能、排泄、脂蛋白结构和功能等。
英文摘要
Using the facultative phototrophic bacterium, Rhodopseudomonas sphaeroides,
we are attempting to understand, at the molecular level, gene control of
the inducible photosynthetic membrane system (ICM). The genetic basis for
ICM expression extends to the: 1. Major bacteriochlorophyll-protein
spectral complexes, cytochromes, ATPase and other ICM-associated enzyme
activities and structural proteins, 2. The pathways for carotenoid and
bacteriochlorophyll synthesis, 3. Phospholipid biosynthetic enzymes and the
movement of phospholipids, and 4. The regulatory systems resulting in
cellular adaptation to changes in oxygen partial pressure and light
intensity, and those mechanisms involved in assembly in an orderly manner,
as well as the structure-function interrelationships of the ICM. It is
proposed that we identify, clone and characterize those genetic regions
relevant to ICM structure and regulation. This study includes, structure
of the DNA region, through restriction endonuclease analysis, DNA
sequencing and in vitro and in vivo characterization of gene expression.
We propose to develop procedures for the selection, isolation and
characterization, both in vivo and in vitro, of mutations which are
relevant to ICM structure and function by employing synthetic
deoxyoligonucleotide and transposon induced mutagenesis. We intend to
derive genetic systems to monitor the expression of these cloned regions in
both homologous and heterologous hosts in order to better understand the
mechanisms of gene control. Such studies will bear directly on the broader
question of the nature of the regulatory signals and their function in
heterologous host systems. Finally, we intend to develop genetic and
pseudogenetic systems for the movement, complementation, expression and
facile handling of genetic regions in R. sphaeroides. We shall attempt to
develop conjugation and transduction systems, cosmids, plasmids, promoter
cloning vehicles, etc., employing classical procaryotic genetic techniques
as well as recombinant DNA technologies. Understanding the functional and
structural implications of photosynthesis is fundamental to the adequate
nutrition and, therefore, health of human populations. Of equal
importance, the structure-function interrelationships of
membrane-associated activities is of critical health importance because of
the nature of health related membrane phenomena, e.g. membrane receptors,
mitochondrial function, excretion, lipoprotein structure and function, etc.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
SIGMA FACTOR IN DNA BINDING AND TRANSCRIPTION INITIATION
-
批准号:6490121
-
项目类别:
-
资助金额:$14.59万
-
财政年份:1998
-
负责人:SAMUEL KAPLAN
-
依托单位:
SIGMA FACTOR IN DNA BINDING AND TRANSCRIPTION INITIATION
-
批准号:6342951
-
项目类别:
-
资助金额:$19.12万
-
财政年份:1998
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENOME COMPLEXITY IN RHODOBACTER SPHAEROIDES 241
-
批准号:2734828
-
项目类别:
-
资助金额:$25.8万
-
财政年份:1997
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENOME COMPLEXITY IN RHODOBACTER SPHAEROIDES 241
-
批准号:6019257
-
项目类别:
-
资助金额:$27.2万
-
财政年份:1997
-
负责人:SAMUEL KAPLAN
-
依托单位:
PEDIATRIC PULMONARY & CARDIOVASCULAR COMPLICATIONS OF VERTICALLY TRANSMITTED HIV
-
批准号:6279565
-
项目类别:
-
资助金额:$1.77万
-
财政年份:1997
-
负责人:SAMUEL KAPLAN
-
依托单位:
PEDIATRIC PULMONARY & CARDIOVASCULAR COMPLICATIONS OF VERTICALLY TRANSMITTED HIV
-
批准号:6249534
-
项目类别:
-
资助金额:$1.8万
-
财政年份:1997
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENOME COMPLEXITY IN RHODOBACTER SPHAEROIDES 241
-
批准号:2024041
-
项目类别:
-
资助金额:$24.58万
-
财政年份:1997
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENOME COMPLEXITY IN RHODOBACTER SPHAEROIDES 241
-
批准号:6180673
-
项目类别:
-
资助金额:$24.17万
-
财政年份:1997
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENETIC ANALYSIS OF BACTERIAL PHOTOSYNTHESIS
-
批准号:3279882
-
项目类别:
-
资助金额:$23.67万
-
财政年份:1989
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENETIC ANALYSIS OF BACTERIAL PHOTOSYNTHESIS
-
批准号:3279875
-
项目类别:
-
资助金额:$23.39万
-
财政年份:1989
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENETIC ANALYSIS OF BACTERIAL PHOTOSYNTHESIS
-
批准号:3279883
-
项目类别:
-
资助金额:$20.73万
-
财政年份:1989
-
负责人:SAMUEL KAPLAN
-
依托单位:
INDUCTION AND BIOGENESIS OF SUBCELLULAR ORGANELLES
-
批准号:3268808
-
项目类别:
-
资助金额:$3.68万
-
财政年份:1989
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENETIC ANALYSIS OF BACTERIAL PHOTOSYNTHESIS
-
批准号:2176244
-
项目类别:
-
资助金额:$24.43万
-
财政年份:1989
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENETIC ANALYSIS OF BACTERIAL PHOTOSYNTHESIS
-
批准号:3279881
-
项目类别:
-
资助金额:$22.75万
-
财政年份:1989
-
负责人:SAMUEL KAPLAN
-
依托单位:
GENETIC ANALYSIS OF BACTERIAL PHOTOSYNTHESIS
-
批准号:3279876
-
项目类别:
-
资助金额:$0.32万
-
财政年份:1989
-
负责人:SAMUEL KAPLAN
-
依托单位:
SMALL INSTRUMENTATION PROGRAM
-
批准号:3524686
-
项目类别:
-
资助金额:$7.46万
-
财政年份:1988
-
负责人:SAMUEL KAPLAN
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
-
批准号:3510337
-
项目类别:
-
资助金额:$0.6万
-
财政年份:1987
-
负责人:SAMUEL KAPLAN
-
依托单位:
OSCILLOSCOPE LASER AMPLIFIER OPTICAL SPECTROMETER
-
批准号:3524398
-
项目类别:
-
资助金额:$6.59万
-
财政年份:1987
-
负责人:SAMUEL KAPLAN
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT
-
批准号:3517852
-
项目类别:
-
资助金额:$25.6万
-
财政年份:1987
-
负责人:SAMUEL KAPLAN
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT
-
批准号:3517851
-
项目类别:
-
资助金额:$24.14万
-
财政年份:1986
-
负责人:SAMUEL KAPLAN
-
依托单位:
海外基金