REGULATION OF EXOGENOUSLY INDUCED OPERON

外源诱导操纵子的调控

• 批准号: 3280191
• 负责人: JEN SHIANG HONG
• 金额: $ 18.26万
• 依托单位: BOSTON BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3280191
• 关键词: DNA binding protein; Escherichia coli; Salmonella typhimurium; bacterial genetics; binding proteins; biological transport; crosslink; gene expression; genetic manipulation; genetic transcription; genetic translation; goats; immunochemistry; membrane proteins; molecular cloning; mutant; nucleic acid probes; nucleic acid sequence; operon; phosphoglycerate; radiotracer; regulatory gene; site directed mutagenesis

The genetic expression of the phosphoglycerate transport system of S. typhimurium is induced by external inducers but not by internal inducers. The long-term objectives of the proposed research are to gentically and biochemically characterize the regulatory machinery involved in gene expression of this transport system and to understand the biochemical and molecular mechanism underlying this novel gene regulation. During the current grant period we dissected the genetic regulatory machinery, determined the DNA sequence, identified gene products and determined the cellular locations of these products. The specific aims of the present proposal are: to isolate, purify and characterize the pgt regulatory proteins; to study the regulatory mechanism in in vitro transcription-translation coupled system in order to determine the functional role of each regulatory proteins with respect to inducer binding, DNA binding and signal transmission; to analyze pgt transcripts and determine pgtP transcription initiation start and DNA binding regions, in order to distinguish two models of regulation and to define the control regions; to isolate constitutive regulatory mutants by genetic selection and site-directed mutagenesis and to characterize the mutant proteins; to study processing of regulatoryp proteins and determine the cleavage sites; and to perform chemical cross-linking experiments to investigate the interaction of the regulatory proteins.

S. 磷酸甘油酸转运系统的基因表达。 鼠伤寒杆菌是由外部诱导物诱导的，但不是由内部诱导物诱导的。 拟议研究的长期目标是温和地、 生物化学表征基因调控机制 该运输系统的表达并了解生化和 这种新型基因调控的分子机制。 期间 当前的资助期我们剖析了基因调控机制， 确定DNA序列，鉴定基因产物并确定 这些产品的蜂窝位置。 当前的具体目标 建议是：分离、纯化和表征 PGT 监管 蛋白质；研究体外调节机制 转录-翻译耦合系统以确定 每种调节蛋白相对于诱导剂的功能作用 结合、DNA结合和信号传递；分析 PGT 转录本 并确定pgtP转录起始起始区和DNA结合区， 为了区分两种监管模式并定义控制 地区；通过遗传选择分离组成型调控突变体 和定点诱变并表征突变蛋白；到 研究调节蛋白的加工并确定切割位点； 并进行化学交联实验来研究 调节蛋白的相互作用。

项目成果

Identification of the products and nucleotide sequences of two regulatory genes involved in the exogenous induction of phosphoglycerate transport in Salmonella typhimurium.

鉴定参与鼠伤寒沙门氏菌磷酸甘油酸转运外源诱导的两个调控基因的产物和核苷酸序列。

• DOI: 10.1128/jb.170.9.4299-4303.1988
• 发表时间: 1988
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Yang,YL;Goldrick,D;Hong,JS
• 通讯作者: Hong,JS

Identification and nucleotide sequence of the activator gene of the externally induced phosphoglycerate transport system of Salmonella typhimurium.

外部诱导的鼠伤寒沙门氏菌磷酸甘油酸转运系统激活基因的鉴定和核苷酸序列。

• DOI: 10.1016/0378-1119(86)90131-9
• 发表时间: 1986
• 期刊: Gene
• 影响因子: 3.5
• 作者: Yu,GQ;Hong,JS
• 通讯作者: Hong,JS