MECHANISM OF REGULATION OF HEAT SHOCK GENE EXPRESSION

胚胎发育、细胞分化或 对新环境的适应都涉及水平的变化 各种基因的表达。我们对这些的理解 因此，流程在很大程度上依赖于我们对 基因调控的分子机制。热休克蛋白 (HSP)基因将被用作研究的模型系统，在 分子水平，在上述几个方面的基因调控 流程。这些基因的表达水平通常很低。 但在较高的温度下被激活，并由许多 细胞应激，及其产物被认为可以保护细胞 抵抗应激损伤。其中一些基因也活跃在 正常发育的不同阶段。 热诱导果蝇等热休克蛋白基因的表达 依赖于监管单位发起人的存在 由(最低限度)HSP基因特异性的两个结合位点组成 转录因子。这一因素，它一直存在 在细胞中，被应激激活，然后结合到调节 单位。早期的发现表明，因子分子结合 到调控单位需要相互作用才能产生促进剂 活动。我们建议(1)继续进行体内和体外研究 定义因子分子之间的相关相互作用 结合到热休克单元，(2)提高抗体 并获得其cdna基因，以确定其性质。 因子分子间的相互作用及其作用机制 因子激活，(3)提纯和鉴定另一因子 可能参与了大脑发育的调节 果蝇hsp23基因(蜕皮激素受体)，以及(4) 描述hsp23的第二种发育调节类型 吉恩。

Processes such as embryonic development, cell differentiation or adaptation to new environments all involve changes in the levels of expression of various genes. Our understanding of these processes is, therefore, critically dependent on our knowledge of the molecular mechanisms of gene regulation. Heat shock protein (hsp) genes will be used as a model system for studying, at the molecular level, aspects of gene regulation in several of the above processes. These genes are typically expressed at very low levels but are activated at elevated temperatures and by a number of cellular stresses, and their products are thought to protect cells against stress damage. Some of these genes are also active at different stages of normal development. Heat-induced expression of Drosophila and other hsp genes appears to depend on the presence in the promoters of a regulation unit consisting (minimally) of two binding sites for a hsp gene-specific transcription factor. This factor, which is continuously present in cells, is activated by stress and then binds to the regulation unit. Earlier findings suggest that the factor molecules binding to the regulation unit need to interact to produce promoter activity. We propose (1) to continue in vivo and in vitro studies to define the relevant interactions between factor molecules binding to the heat shock unit, (2) to raise antibodies against such a factor and to obtain its cDNA gene to define the nature of the interactions between factor molecules and the mechanism of factor activation, (3) to purify and characterize another factor likely to be involved in the developmental regulation of the Drosophila hsp23 gene (ecdysterone receptor), and (4) to characterize a second type of developmental regulation of the hsp23 gene.