TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA SELECTI
mRNA 选择对基因表达的翻译控制
基本信息
- 批准号:3283541
- 负责人:
- 金额:$ 7.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1983
- 资助国家:美国
- 起止时间:1983-12-01 至 1986-11-30
- 项目状态:已结题
- 来源:
- 关键词:cell free system density gradient ultracentrifugation developmental genetics early embryonic stage embryogenic cleavage fertilization gel electrophoresis gene expression genetic manipulation genetic translation germ cells immunochemistry messenger RNA nonmammalian vertebrate embryology ribosomes saltwater environment
项目摘要
Qualitative control of translation is an important mechanism for changing
the relative abundancies of different proteins. It is achieved by
selective regulation of mRNA binding to ribosomes and is a significant
feature of the control of gene expression in animal cells. Such control is
observed in the shut-off of host protein synthesis by viruses, in the
maintenance of the differentiated state, in the response of cells to
heat-shock and in the utilization of stored maternal mRNA during early
development. Not only is this control of translation a means of short-term
regulation of gene expression, but increasing evidence suggests that it may
provide fine-tuning on transcription by feed-back inhibition. The
mechanism whereby qualitative control is exerted is currently not
understood. Conceivable mechanisms include:
a) Non-specific changes in the overall rate of protein synthesis caused by
changes in the availability of mRNAs, activated small ribosomal subunits,
or initiation factors.
b) Specific inhibition of mRNA binding to ribosomes by inhibitory factors
or product feedback.
c) Specific promotion of mRNA binding by stimulatory factors.
The present proposal undertakes to study qualitative translational control
using histone synthesis in sea urchin eggs and cleavage-stage embryos as a
model system. Whether one or all of the above mechanisms are operative
will be determined. The sea urchin system is chosen because histone
synthesis is known to be subject to translational control and because there
are indications that the system exhibits multiple types of mRNA selection
processes. Different histone variants are synthesized at different
developmental stages, suggestive of a role of the variants in the
regulation of gene expression. In view of this, an understanding of
translational control of the histone gene family will increase our
comprenension of the regulatory mechanisms operative on gene expression
during development.
翻译的质量控制是一种重要的改变机制
不同蛋白质的相对丰度。 它是通过以下方式实现的:
选择性调节mRNA与核糖体的结合,
控制动物细胞中基因表达的特征。 这种控制
在病毒关闭宿主蛋白质合成中观察到,
维持分化状态,在细胞对
热休克和在利用储存的母体mRNA在早期
发展 这种对翻译的控制不仅是一种短期的
调节基因表达,但越来越多的证据表明,它可能
通过反馈抑制对转录进行微调。 的
质量控制机制目前还没有
明白 可设想的机制包括:
a)蛋白质合成总速率的非特异性变化,
mRNA可用性的变化,活化的小核糖体亚基,
或启动因素。
B)抑制因子对mRNA与核糖体结合的特异性抑制
或产品反馈。
c)通过刺激因子特异性促进mRNA结合。
本建议致力于研究定性翻译控制
利用海胆卵和卵裂期胚胎中的组蛋白合成,
模型系统。 上述机制之一或全部是否有效
将被确定。 选择海胆系统是因为
已知合成受翻译控制,因为
表明该系统表现出多种类型的mRNA选择
流程. 不同的组蛋白变体是在不同的合成条件下合成的。
发展阶段,暗示的作用的变体,
基因表达的调控。 有鉴于此,
组蛋白基因家族的翻译控制将增加我们的
基因表达调控机制的综述
在发展过程中。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ROSEMARY JAGUS其他文献
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{{ truncateString('ROSEMARY JAGUS', 18)}}的其他基金
Translation regulation of gene expression in toxic dinoflagellates
有毒甲藻基因表达的翻译调控
- 批准号:
8707455 - 财政年份:2012
- 资助金额:
$ 7.97万 - 项目类别:
Translation regulation of gene expression in toxic dinoflagellates
有毒甲藻基因表达的翻译调控
- 批准号:
8388403 - 财政年份:2012
- 资助金额:
$ 7.97万 - 项目类别:
Translation regulation of gene expression in toxic dinoflagellates
有毒甲藻基因表达的翻译调控
- 批准号:
8550056 - 财政年份:2012
- 资助金额:
$ 7.97万 - 项目类别:
CONTROL OF EIF-2 ACTIVITY IN REGULATION OF TRANSLATION
翻译调节中 EIF-2 活性的控制
- 批准号:
3284080 - 财政年份:1984
- 资助金额:
$ 7.97万 - 项目类别:
CONTROL OF EIF-2 ACTIVITY IN REGULATION OF TRANSLATION
翻译调节中 EIF-2 活性的控制
- 批准号:
3284079 - 财政年份:1984
- 资助金额:
$ 7.97万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA SELECTI
mRNA 选择对基因表达的翻译控制
- 批准号:
3283542 - 财政年份:1983
- 资助金额:
$ 7.97万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA
mRNA 对基因表达的翻译控制
- 批准号:
3283544 - 财政年份:1983
- 资助金额:
$ 7.97万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA
mRNA 对基因表达的翻译控制
- 批准号:
3283540 - 财政年份:1983
- 资助金额:
$ 7.97万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA
mRNA 对基因表达的翻译控制
- 批准号:
3283545 - 财政年份:1983
- 资助金额:
$ 7.97万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA
mRNA 对基因表达的翻译控制
- 批准号:
3283543 - 财政年份:1983
- 资助金额:
$ 7.97万 - 项目类别:
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- 批准号:
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