TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA
mRNA 对基因表达的翻译控制
基本信息
- 批准号:3283545
- 负责人:
- 金额:$ 12.28万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1983
- 资助国家:美国
- 起止时间:1983-12-01 至 1992-06-30
- 项目状态:已结题
- 来源:
- 关键词:Nematoda adenosine triphosphate antibody binding proteins calcium cell free system chemical binding chemical structure function crosslink density gradient ultracentrifugation developmental genetics early embryonic stage embryogenesis fertilization gel electrophoresis gene expression genetic manipulation genetic transcription genetic translation germ cells histones hydrolysis immunochemistry messenger RNA nonmammalian vertebrate embryology nuclease nucleic acid probes protein biosynthesis protein kinase protein structure radiotracer ribonucleoproteins ribosomes saltwater environment sea urchins synthetic nucleic acid
项目摘要
During oogenesis and maturation of the sea urchin egg, large
stores of mRNA are accumulated for subsequent use during early
embryogenesis. Utilization of stored maternal mRNA occurs at a
very low rate prior to fertilization. After fertilization, dramatic
changes in mRNA availability and recruitment occur, allowing
rapid increases in the synthetic rate of many proteins during early
cleavage. Changes have been documented in both overall rates of
maternal mRNA utilization and in the selection of particular
mRNA species at different developmental stages. Our previous
studies have allowed the development of cell-free translation
systems from eggs and embryos that reproduce the overall
differences observed in the intact system.
This investigation proposes to utilize these cell-free systems, in
conjunction with other approaches, to identify the cellular
components involved in the utilization of maternal mRNA. Using
the cell-free systems an inhibitor of eIF-4F activity has been
discovered in the unfertilized egg, that decreases gradually in
activity over the first 2-3 hr after fertilization. The identity and
nature of the eIF-4F inhibitory activity will be characterized.
The mechanism by which this activity inhibits the initiation
sequence will also be investigated. The manner in which the
inhibitor is inactivated after fertilization will be explored. To
complement these studies, a systematic search for
developmentally regulated mRNA-interactive proteins will be
undertaken, using a protein blotting and in vitro binding
technique, with capped 32p-labelled mRNA's synthesized in vitro.
在海胆卵的卵子发生和成熟过程中,
mRNA的储存积累起来,以供早期使用。
胚胎发生 储存的母体mRNA的利用发生在
在受精前的低水平。 受精后,戏剧性的
mRNA的可用性和募集发生变化,
在早期,许多蛋白质的合成速率迅速增加,
乳沟 变化已记录在两个总比率,
母体mRNA的利用和在选择特定的
不同发育阶段的mRNA种类。 我们以前的
研究已经允许无细胞翻译的发展,
从卵子和胚胎中提取的系统,
在完整系统中观察到的差异。
本研究提出利用这些无细胞系统,
结合其他方法来识别细胞
参与利用母体mRNA的组分。 使用
在无细胞系统中,eIF-4F活性的抑制剂已经被
在未受精卵中发现,
受精后2-3小时内的活性。 身份和
将表征eIF-4F抑制活性的性质。
这种活动抑制启动的机制
序列也将被研究。 的方式
抑制剂在受精后失活的可能性将被探索。 到
作为对这些研究的补充,
发育调控的mRNA相互作用蛋白将是
采用蛋白质印迹和体外结合
技术,在体外合成加帽的32 P标记的mRNA。
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Mechanism of action of developmentally regulated sea urchin inhibitor of eIF-4.
eIF-4 发育调控海胆抑制剂的作用机制。
- DOI:10.1002/dvg.1020140603
- 发表时间:1993
- 期刊:
- 影响因子:0
- 作者:Jagus,R;Huang,W;Hiremath,LS;Stern,BD;Rhoads,RE
- 通讯作者:Rhoads,RE
Increase in eukaryotic initiation factor 2B activity following fertilization reflects changes in redox potential.
受精后真核起始因子 2B 活性的增加反映了氧化还原电位的变化。
- DOI:
- 发表时间:1991
- 期刊:
- 影响因子:0
- 作者:Akkaraju,GR;Hansen,LJ;Jagus,R
- 通讯作者:Jagus,R
Use of nonreducing SDS-PAGE for monitoring renaturation of recombinant protein synthesis initiation factor, eIF-4 alpha.
使用非还原 SDS-PAGE 监测重组蛋白合成起始因子 eIF-4 α 的复性。
- DOI:10.1006/prep.1993.1041
- 发表时间:1993
- 期刊:
- 影响因子:1.6
- 作者:Stern,BD;Wilson,M;Jagus,R
- 通讯作者:Jagus,R
Changes in rates of protein synthesis and eukaryotic initiation factor-4 inhibitory activity in cell-free translation systems of sea urchin eggs and early cleavage stage embryos.
海胆卵和早期卵裂阶段胚胎的无细胞翻译系统中蛋白质合成速率和真核起始因子 4 抑制活性的变化。
- DOI:
- 发表时间:1992
- 期刊:
- 影响因子:0
- 作者:Jagus,R;Huang,WI;Hansen,LJ;Wilson,MA
- 通讯作者:Wilson,MA
Inhibitor of translational initiation in sea urchin eggs prevents mRNA utilization.
海胆卵翻译起始抑制剂可阻止 mRNA 的利用。
- DOI:
- 发表时间:1987
- 期刊:
- 影响因子:0
- 作者:Hansen,LJ;Huang,WI;Jagus,R
- 通讯作者:Jagus,R
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ROSEMARY JAGUS其他文献
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{{ truncateString('ROSEMARY JAGUS', 18)}}的其他基金
Translation regulation of gene expression in toxic dinoflagellates
有毒甲藻基因表达的翻译调控
- 批准号:
8707455 - 财政年份:2012
- 资助金额:
$ 12.28万 - 项目类别:
Translation regulation of gene expression in toxic dinoflagellates
有毒甲藻基因表达的翻译调控
- 批准号:
8388403 - 财政年份:2012
- 资助金额:
$ 12.28万 - 项目类别:
Translation regulation of gene expression in toxic dinoflagellates
有毒甲藻基因表达的翻译调控
- 批准号:
8550056 - 财政年份:2012
- 资助金额:
$ 12.28万 - 项目类别:
CONTROL OF EIF-2 ACTIVITY IN REGULATION OF TRANSLATION
翻译调节中 EIF-2 活性的控制
- 批准号:
3284080 - 财政年份:1984
- 资助金额:
$ 12.28万 - 项目类别:
CONTROL OF EIF-2 ACTIVITY IN REGULATION OF TRANSLATION
翻译调节中 EIF-2 活性的控制
- 批准号:
3284079 - 财政年份:1984
- 资助金额:
$ 12.28万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA SELECTI
mRNA 选择对基因表达的翻译控制
- 批准号:
3283542 - 财政年份:1983
- 资助金额:
$ 12.28万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA
mRNA 对基因表达的翻译控制
- 批准号:
3283544 - 财政年份:1983
- 资助金额:
$ 12.28万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA
mRNA 对基因表达的翻译控制
- 批准号:
3283540 - 财政年份:1983
- 资助金额:
$ 12.28万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA SELECTI
mRNA 选择对基因表达的翻译控制
- 批准号:
3283541 - 财政年份:1983
- 资助金额:
$ 12.28万 - 项目类别:
TRANSLATIONAL CONTROL OF GENE EXPRESSION BY MRNA
mRNA 对基因表达的翻译控制
- 批准号:
3283543 - 财政年份:1983
- 资助金额:
$ 12.28万 - 项目类别:
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