OXIDATION CHEMISTRY OF INDOLES

吲哚的氧化化学

• 批准号: 3281126
• 负责人: GLENN DRYHURST
• 金额: $ 7.64万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-15 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3281126
• 关键词: 5 hydroxytryptophan; electrochemistry; indoles; neurotoxins; oxidation; quinones; serotonin; tryptophan

The specific aim of this project is to employ electrochemical and related analytical techniques to investigate the oxidation chemistry of a group of naturally occurring or otherwise biologically significant 3-substituted indoles. Among the compounds which will be investigated are tryptophan, 5-hydroxytryptamine, 5,6- and 5-7- dihydroxytryptamine, indole-3-acetic acid and various metabolities of these species. There is abundant literature evidence that these compounds undergo many oxidation reactions in living systems yet the mechanisms and products of these processes are not understood. In order to begin to understand the chemistry involved in such reactions a basic, fundamental study of the oxidation chemistry is required. It is believed that electrochemical and associated analytical methodologies provide the best techniques to study this oxidation chemistry. Our previous investigations have clearly shown that electrochemical studies of the oxidation and related chemistry of biological molecules can provide valuable insights into the biochemical oxidation mechanisms. It is expected that the planned studies on the oxidation chemistry of indoles will provide even more valuable information. It is planned to employ electrochemical and related techniques to define the redox potentials associated with the oxidation reactions of indoles, to chemically and physically characterize intermediates and to identify and quantitate products. Furthermore, it is hoped to develop an understanding of the interactions of putative oxidative intermediates such as quinones and quinoneimines with species commonly found in tissue. The ultimate goal of the proposed work is to provide a rational and systematic basis for understanding the oxidation chemistry of biologically significant indoles. This is turn should provide the basis for understanding the oxidation chemistry of indoles in living systems.

该项目的具体目标是采用电化学和相关的 分析技术来研究一组氧化化学 天然存在的或具有生物学意义的3-取代的 吲哚。 在将要研究的化合物中有色氨酸， 5-羟色胺，5，6-和5-7-二羟色胺，吲哚-3-乙酸 酸和这些物种的各种代谢。 有大量 文献证据表明这些化合物经历许多氧化反应 然而，这些过程的机制和产物， 不理解。 为了开始理解 这些反应是氧化化学的一个基本的、基础的研究， 必需的. 据信，电化学和相关的分析， 方法学提供了研究这种氧化的最佳技术 化学. 我们之前的调查清楚地表明， 的氧化及相关化学的电化学研究 生物分子可以提供有价值的见解， 氧化机制 预计计划中的关于 吲哚的氧化化学将提供更有价值的 信息. 计划采用电化学和相关技术， 技术来确定与氧化相关的氧化还原电位 吲哚的化学和物理特性 中间体，并识别和定量产品。 再者是 希望能对假定的氧化性 中间体如醌和醌亚胺， 在组织中发现。 拟议工作的最终目标是提供一个 合理的和系统的基础，了解氧化化学的 具有生物学意义的吲哚。 这为转应提供了依据 以了解生命系统中吲哚的氧化化学。