TANDEM MASS SPECTROMETER FOR BIOMOLECULAR ANALYSIS

俄克拉何马大学化学与生物化学系提议购买一台串联质谱仪，该质谱仪具有大气压电离（API）源，能够同时进行电喷雾电离（ESI）和大气压化学电离（APCI）。该仪器将成为化学和生物化学系已建立的分析服务中心的一部分。这种新仪器将支持俄克拉荷马大学越来越多的生物无机、生物有机和生物化学研究小组的活动。该仪器将主要由本提案中包括的美国国立卫生研究院支持的用户组使用，尽管其他用户可能会提交样品以供分析。该质谱仪将满足各种项目的质量分析需求，包括研究异常脑化学中生物胺类神经递质的异常氧化代谢物的作用，细菌铁载体的鉴定，含Ru或Os的金属卟啉的物理结合供体的测定，蛋白质和RNA核苷酸相互作用的结合位点的测定。并对酶的自杀失活机理和化学计量学进行了研究。

The Department of Chemistry and Biochemistry at the University of Oklahoma proposes the acquisition of a tandem mass spectrometer with an atmospheric pressure ionization (API) source capable of both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). The instrument will become a part of the established Analytical Services Center in the Chemistry and Biochemistry Department. This new instrument will support the activities of a growing number of bioinorganic bioorganic and biochemical research groups at the University of Oklahoma. Primary use of this instrument will be by the NIH-supported user group included in this proposal, although other users may submit samples for analysis of a time-available basis. This mass spectrometer will fill the mass analysis needs for a variety of projects including investigations into the roles of unusual oxidative metabolites of biogenic amine neurotransmitters in aberrant brain chemistry, identification of bacterial siderophores, determination of physical binding donors to metalloporphyrins containing Ru or Os, determination of the binding site for interaction of protein and RNA nucleotide, and the determination of the mechanism and stoichiometry of suicide inactivation of enzymes.