NMR STUDIES OF METABOLIC PATHWAYS IN VIVO AND IN VITRO

体内和体外代谢途径的 NMR 研究

• 批准号: 3281595
• 负责人: ALASTAIR I SCOTT
• 金额: $ 19.22万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-03-01 至 1988-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3281595
• 关键词: Fungi; Krebs' cycle; bacteria; biotransformation; cell free system; cephalosporins; chemical reaction; chemical synthesis; cholesterol; chymotrypsin; clinical biomedical equipment; cryoscience; eicosanoid metabolism; erythrocytes; fatty acid biosynthesis; fatty acid synthase; fermentation; fibroblasts; gluconeogenesis; glycolysis; human tissue; luciferin monooxygenase; metalloenzyme; methionine; methylmalonyl coA epimerase; neoplastic cell; noninvasive diagnosis; nuclear magnetic resonance spectroscopy; nutrition related tag; o aminobenzoate; pancreatic ribonuclease; papain; penicillins; plants; prostaglandin endoperoxide synthase; purine /pyrimidine metabolism; radiopharmacology; squalene; stable isotope diagnosis; steroid biosynthesis; tetracyclines; tissue /cell culture; trypsin; vitamin B12 compound; vitamin biosynthesis

Development of techniques using wide-bore, high-field (300-500 MHz), high-resolution NMR spectroscopy to study metabolic processes in living systems and in vitro will be undertaken. The use of multiply labeled substrates (13C, 15N, 2H) prepared by regiospecific synthesis will be of value in assigning chemical shifts to unknown and short-lived species. It is proposed to use cryoenzymology and flow cells to study the flux of carbon, hydrogen and nitrogen in biochemical reactions, to identify hitherto undetected species and to devise non-invasive methods for the analysis of microgram quantities of biologically important molecules during metabolism in mammalian, bacterial and plant cells, as well as whole, perfused organs and insects.

使用宽孔，高田（300-500 MHz）开发技术， 高分辨率的NMR光谱学研究了生活中的代谢过程 系统和体外将进行。 使用乘以标记的 由Regiospific合成制备的底物（13C，15N，2H）将为 将化学转移分配给未知物种和短暂物种的价值。 它 提议使用冷冻酶学和流动细胞来研究 生化反应中的碳，氢和氮，以鉴定 迄今未发现的物种，并设计非侵入性方法 分析微克在生物学上重要的分子的分析 哺乳动物，细菌和植物细胞以及整体的代谢 灌注器官和昆虫。