GLUCONEOGENESIS & KREBS CYCLE FLUXES IN HUMANS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A basic question in the pathogenesis of type 2 diabetes (T2DM) is the mechanism of excessive glucose production. In recent years multiple studies have assessed gluconeogenesis (the sum of glucose originating from carbon skeletons in the TCA cycle and glycerol) versus glycogenolysis after an overnight fast. The goal of this project is to refine the technologies and concepts developed in Cores 1 and 2 for routine applications in humans. In addition to continuing our work with conventional 2H and 13C NMR for analysis of plasma and urine under various metabolic conditions, we have initiated studies at 3T and 7T for measurement of intramyocellular lipids and glycogen content in skeletal muscle of humans.