NMR STUDIES OF METABOLIC PATHWAYS IN VIVO AND IN VITRO

体内和体外代谢途径的 NMR 研究

• 批准号: 3281597
• 负责人: ALASTAIR I SCOTT
• 金额: $ 21.63万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-03-01 至 1988-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3281597
• 关键词: Fungi; Krebs' cycle; bacteria; biotransformation; cell free system; cephalosporins; chemical reaction; chemical synthesis; cholesterol; chymotrypsin; clinical biomedical equipment; cryoscience; deuterium; eicosanoid metabolism; erythrocytes; fatty acid biosynthesis; fatty acid synthase; fermentation; fibroblasts; gluconeogenesis; glycolysis; human tissue; luciferin monooxygenase; metalloenzyme; methionine; methylmalonyl coA epimerase; neoplastic cell; noninvasive diagnosis; nuclear magnetic resonance spectroscopy; nutrition related tag; o aminobenzoate; pancreatic ribonuclease; papain; penicillins; plants; prostaglandin endoperoxide synthase; purine /pyrimidine metabolism; radiopharmacology; squalene; stable isotope diagnosis; steroid biosynthesis; tetracyclines; tissue /cell culture; trypsin; vitamin B12 compound; vitamin biosynthesis

Development of techniques using wide-bore, high-field (300-500 MHz), high-resolution NMR spectroscopy to study metabolic processes in living systems and in vitro will be undertaken. The use of multiply labeled substrates (13C, 15N, 2H) prepared by regiospecific synthesis will be of value in assigning chemical shifts to unknown and short-lived species. It is proposed to use cryoenzymology and flow cells to study the flux of carbon, hydrogen and nitrogen in biochemical reactions, to identify hitherto undetected species and to devise non-invasive methods for the analysis of microgram quantities of biologically important molecules during metabolism in mammalian, bacterial and plant cells, as well as whole, perfused organs and insects.

发展使用宽口径、高场（300-500兆赫）、 高分辨率核磁共振波谱学研究生命代谢过程 系统和体外将进行。 多重标记的使用 通过区域特异性合成制备的底物（13 C，15 N，2 H）将是 在分配化学位移未知和短命物种的价值。 它 建议使用低温酶学和流动池来研究 生物化学反应中的碳、氢和氮， 迄今未被发现的物种，并制定非侵入性的方法， 分析微克数量的生物学重要分子， 在哺乳动物、细菌和植物细胞中的代谢，以及整体， 灌注的器官和昆虫。