Sussex Crystallization Platform for Bioscience discovery

用于生物科学发现的苏塞克斯结晶平台

• 批准号: BB/W02019X/1
• 负责人: Erika Mancini
• 金额: $ 91.18万
• 依托单位: University of Sussex
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW02019X%2F1
• 关键词: Sussex; Crystallization; Platform; Bioscience; discovery

We are seeking funding to purchase new state-of-the-art crystallization equipment for the School of Life Science's crystallisation facility to enable us to visualise and study the way in which large molecular machines control the replication, survival and growth of human cells and how the protein complexes produced by microbes exploit and disrupt human cell systems. Central to this work is the ability to capture these proteins and protein complexes in solid crystal form to allow us to take a 'snapshop' using X-rays to determine their three-dimensional shape and detailed atomic structure and how this is altered in different conditions.Financial support is sought for the purchase of a state-of-the-art crystallisation platform to enable us to do this faster, more efficiently and more effectively. The platform platform has integrated software and comprises a crystallization robot, an advanced large capacity automated crystal imaging system and a next-generation liquid handler for crystallization screen building. The equipment will be housed in the crystallization facility at Sussex which has been highly successful in advancing our bioscience discovery research portfolio on the structure and function of macromolecular assemblies; the large number of outputs generated from this research has led to Sussex being internationally recognised as a centre of excellence for structural biology. Building on these discoveries, the facility has also enabled the growth of our translational research activities with the Sussex Drug Discovery Centre and resulted in a rapid expansion of the number of structure-led compound and drug discovery projects initiated across the School. This new equipment will enable us to move at pace with our programmes of bioscience projects and to generate higher quality data and experimental outcomes.

我们正在寻求资金，为生命科学学院的结晶设施购买新的最先进的结晶设备，使我们能够可视化和研究大型分子机器控制人类细胞复制，生存和生长的方式，以及微生物产生的蛋白质复合物如何利用和破坏人类细胞系统。这项工作的核心是能够以固体晶体形式捕获这些蛋白质和蛋白质复合物，使我们能够使用X射线进行“快照”，以确定它们的三维形状和详细的原子结构，以及这些结构在不同条件下如何发生变化。我们正在寻求财政支持，以购买最先进的结晶平台，使我们能够更快，更高效，更有效地完成这项工作。该平台具有集成软件，包括结晶机器人、先进的大容量自动晶体成像系统和用于结晶筛构建的下一代液体处理器。该设备将被安置在苏塞克斯的结晶设施中，该设施在推进我们关于大分子组装体的结构和功能的生物科学发现研究组合方面非常成功;这项研究产生的大量产出使苏塞克斯成为国际公认的结构生物学卓越中心。在这些发现的基础上，该设施还使我们的转化研究活动与苏塞克斯药物发现中心的增长，并导致整个学校发起的结构主导的化合物和药物发现项目的数量迅速扩大。这一新设备将使我们能够与我们的生物科学项目方案保持同步，并产生更高质量的数据和实验结果。