Testing the role of sleep in homeostatic plasticity

测试睡眠在稳态可塑性中的作用

• 批准号: BB/X000273/1
• 负责人: Andrew Lin
• 金额: $ 55.63万
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FX000273%2F1
• 关键词: Testing; role; sleep; homeostatic; plasticity

We spend 1/3 of our lives asleep, unconscious and disconnected from the world. It seems like a waste of time, yet all animals sleep, so it seems sleep must fulfill some important biological purpose. What might that purpose be?Sleep improves memory and our minds work less well when we're sleep-deprived, suggesting that sleep has a neurological function. Indeed, one hypothesis posits that sleep is required for the brain to maintain stable levels of activity. This is important because neurons in the brain are all connected: when a neuron fires an electrical impulse, it sends chemical signals to other neurons that either excite them (make them fire) or inhibit them (stop them from firing). If excitation and inhibition become imbalanced, a neural network can spiral out of control into a seizure (too much excitation) or silence (too much inhibition). Yet our brains are constantly changing as we learn based on sensory experience. To stop these changes from unbalancing excitation and inhibition, the brain readjusts neurons and their connections to compensate for the changes and restore stable activity levels, a process called "homeostatic plasticity". It's thought that this process might be best carried out during sleep, a time of inactivity with little sensory input - much as shops do inventory checks after hours.Although this idea has much supporting evidence, it's not clear exactly how sleep is involved in homeostatic plasticity. First, is sleep specifically required for particular *kinds* of homeostatic plasticity? One influential hypothesis posits that connections between neurons are mainly strengthened when we're awake, and weakened when we're asleep. This idea is supported by much, but not all, evidence. Could sleep's role in homeostatic plasticity be governed by a different logic? For example, perhaps sleep is important for adjusting the strength of connections between neurons but not neurons' own intrinsic ability to be excited by other neurons ('excitability'), or for adjusting the activity of excitatory but not inhibitory neurons. Second, by what molecular mechanisms does sleep influence homeostatic plasticity? Two interesting candidates are "reactive oxygen species" (byproducts of metabolism that can be dangerous yet also play important signaling roles) and levels of certain synaptic proteins (molecules that help neurons signal to each other). Each one is regulated by sleep and plays a role in homeostatic plasticity. Could one or both be a common nexus by which sleep influences homeostatic plasticity?We will address these questions using the olfactory system of the fruit fly Drosophila. Like humans, flies sleep, and we have developed a new model system for studying homeostatic plasticity in the intact brain in flies. Here, neurons called "Kenyon cells" excite, and are inhibited by, a neuron called "APL". If we artificially activate APL for 4 days (producing excess inhibition), the circuit compensates for the perturbation, which is revealed as higher activity in Kenyon cells when we lift the artificially imposed excess inhibition. This effect arises both because APL becomes less active and because Kenyon cells get more excitation, and it requires sleep: it's reduced when we stop flies from sleeping, and it's enhanced when we use a genetic trick to force them to sleep extra.We will test what kinds of homeostatic plasticity sleep is required for, by testing whether sleep is required for (1) a variety of forms of homeostatic plasticity (e.g., excess excitation from Kenyon cells, excess exposure to natural odours) and (2) different possible underlying cellular mechanisms (e.g., changing connection strength between neurons or intrinsic excitability). We will test *how* sleep modulates homeostatic plasticity by measuring and manipulating reactive oxygen species and synaptic protein levels in normal and sleep-deprived flies and testing how this affects homeostatic plasticity.

我们一生中有三分之一的时间在沉睡、无意识和与世隔绝的状态下度过。这看起来像是浪费时间，但所有的动物都会睡觉，所以睡眠似乎必须满足一些重要的生物学目的。这可能是为了什么？睡眠改善记忆，当我们被剥夺睡眠时，我们的大脑工作得不太好，这表明睡眠具有神经功能。事实上，有一种假设认为，大脑需要睡眠才能保持稳定的活动水平。这一点很重要，因为大脑中的神经元都是相互联系的：当一个神经元发出电脉冲时，它会向其他神经元发送化学信号，这些信号要么刺激它们(使它们开火)，要么抑制它们(阻止它们放电)。如果兴奋和抑制变得不平衡，神经网络可能会失控，导致癫痫发作(过度兴奋)或沉默(过度抑制)。然而，随着我们根据感官经验进行学习，我们的大脑也在不断地变化。为了阻止这些变化不平衡的兴奋和抑制，大脑重新调整神经元及其连接，以补偿这些变化并恢复稳定的活动水平，这一过程被称为“自我平衡可塑性”。人们认为，这一过程最好在睡眠期间进行，这段时间不活动，感觉输入很少--就像商店在下班后进行库存检查一样。尽管这一想法有很多支持证据，但还不清楚睡眠是如何参与体内平衡可塑性的。首先，睡眠是不是特定“种类”的动态平衡可塑性所必需的？一个有影响力的假说认为，当我们清醒时，神经元之间的联系主要是加强的，而当我们睡着时，神经元之间的联系主要是减弱的。这一观点得到了许多(但不是全部)证据的支持。睡眠在体内平衡可塑性中的作用可以用不同的逻辑来支配吗？例如，也许睡眠对调节神经元之间联系的强度很重要，但对神经元自身被其他神经元兴奋的内在能力(兴奋性)或调节兴奋性神经元的活动而不是抑制性神经元的活动来说，睡眠是重要的。第二，睡眠通过什么分子机制影响体内平衡可塑性？两个有趣的候选物种是“活性氧种”(代谢的副产物，可能危险，但也起着重要的信号作用)和某些突触蛋白的水平(帮助神经元相互传递信号的分子)。每一种都受到睡眠的调节，并在体内平衡可塑性中发挥作用。睡眠可能是影响体内平衡可塑性的共同纽带吗？我们将利用果蝇的嗅觉系统来解决这些问题。和人类一样，苍蝇也会睡觉，我们已经开发了一种新的模型系统来研究苍蝇完整大脑中的自我平衡可塑性。在这里，被称为“凯尼恩细胞”的神经元兴奋，但被称为“APL”的神经元抑制。如果我们人工激活APL 4天(产生过度抑制)，电路就会补偿这种扰动，当我们解除人为施加的过度抑制时，这种扰动在Kenyon细胞中表现为更高的活性。这种效应的产生既是因为APL变得不那么活跃，也是因为Kenyon细胞获得了更多的兴奋，它需要睡眠：当我们阻止苍蝇睡觉时，它会减少，当我们使用基因技巧迫使它们睡得更远时，它会增强。我们将通过测试是否需要睡眠来测试哪种类型的自我平衡可塑性需要睡眠，通过测试(1)各种形式的自我平衡可塑性(例如，来自Kenyon细胞的过度兴奋，对自然气味的过度暴露)和(2)不同可能的潜在细胞机制(例如，改变神经元之间的连接强度或内在的兴奋性)。我们将通过测量和控制正常和睡眠不足的果蝇的活性氧和突触蛋白水平，并测试这如何影响稳态可塑性，来测试睡眠是如何调节稳态可塑性的。

项目成果

Neuroscience: Hacking development to understand sensory discrimination

神经科学：通过黑客开发来理解感觉辨别

• DOI: 10.1016/j.cub.2023.06.072
• 发表时间: 2023
• 期刊: Current Biology
• 影响因子: 9.2
• 作者: Lin A