MOLECULAR BIOLOGY OF MITOSIS IN ASPERGILLUS

理解有丝分裂，它的调节和它的机制是核心， 许多医学领域涉及细胞生长和分裂，例如， 癌症、胚胎发育和人类遗传学。 的目标 这个项目是一个更好地了解力学和调节， 核部门 当前的目标是识别和描述 利用DNA参与核分裂的特定基因的产物 介导的转化，以补充特定的有丝分裂突变， 构巢曲霉 基因将被克隆、测序， 其特征在于杂交选择和互补翻译 信使RNA。 这些基因的蛋白质产物的抗体将被 制备并用于定位细胞中的基因产物。

An understanding of mitosis, its regulation and its mechanics is central to many areas of medicine having to do with cell growth and division, e.g., cancer, embryological development and human genetics. The objective of this project is a better understanding of the mechanics and regulation of nuclear division. The immediate goal is to identify and characterize the products of specific genes involved in nuclear division by using DNA mediated transformation to complement specific mitotic mutations in Aspergillus nidulans. Genes will be cloned, sequenced and their products characterized by hybrid selection and translation of complementary messenger RNA's. Antibodies to the protein products of these genes will be prepared and used to localize the gene products in cells.