LIVING PROSTHETIC SKIN

活体假皮肤

• 批准号: 3287121
• 负责人: JOHN F HANSBROUGH
• 金额: $ 34.43万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-02-01 至 1988-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3287121
• 关键词: aminoacid; biomaterial development /preparation; biopsy; biotechnology; burn therapy; cell differentiation; clone cells; growth factor; growth media; histocompatibility antigens; hormones; human tissue; keratinocyte; mixed lymphocyte reaction test; monoclonal antibody; mucopolysaccharides; skin transplantation; surface antigens; vitamins

The objective of the proposed research is to develop a living replacement of skin that can be used in the treatment of major burns and skin losses. New methods have recently been developed in the laboratory of one of the co-investigators for convenient and rapid growth in defined medium of human epidermal keratinocytes, and for control of keratinocyte differentiation in culture. In addition, a slowly biodegradable synthetic dermal membrane composed of crosslinked collagen and glycosaminoglycans has recently been described in the literature for use in treating major burns. The overall research plan is to establish cultures of epidermal keratinocytes from a small skin biopsy, expand the cells in culture, coat the membrane with cultured keratinocytes, induce stratification of the keratinocytes and terminal differentiation of the outer layer, and then use the living artificial skin that has been generated for grafting. The cultures will be carefully monitored at all stages to verify absence of malignant transformation and undesirable antigenic changes. An interdisciplinary research team of basic scientists and physicians has been assembled with all of the needed expertise in cell culture, dermatology, biochemistry, oncology and clinical management of burns needed for this project. We anticipate that this procedure will allow cells from a small amount of a burn patient's unburned skin to be expanded rapidly in number and used to prepare a graft that can then be placed permanently over a large burned area with minimum risk of rejection. Such a procedure should greatly reduce total treatment time for patients with burns over a large area of the body, reduce the amount of donor tissue required for grafting, and increase the chances of saving very severely burned patients.

拟议研究的目标是开发一种活体替代品 可用于治疗严重烧伤和皮肤缺损的皮肤。 最近在其中一个实验室开发了新方法 共同研究者在人类特定培养基中方便快速地生长 表皮角质形成细胞，并用于控制角质形成细胞分化 文化。 此外，可缓慢生物降解的合成真皮膜 最近，由交联胶原蛋白和糖胺聚糖组成的 文献中描述了用于治疗严重烧伤的用途。 整体 研究计划是建立表皮角质形成细胞的培养物 小皮肤活检，扩大培养物中的细胞，用 培养角质形成细胞，诱导角质形成细胞分层 外层终末分化，然后利用活体 为移植而生成的人造皮肤。 这些文化将是 在各个阶段都进行仔细监测，以验证是否存在恶性疾病 转化和不良抗原变化。 跨学科 由基础科学家和医生组成的研究团队已组建 细胞培养、皮肤病学、生物化学、 该项目所需的肿瘤学和烧伤临床管理。 我们 预计该程序将允许细胞从少量 烧伤患者未烧伤的皮肤数量迅速扩大并用于 准备移植物，然后将其永久放置在大面积烧伤处 被拒绝风险最小的区域。 这样的程序应该大大 减少大面积烧伤患者的总治疗时间 身体，减少移植所需的供体组织数量，以及 增加拯救严重烧伤患者的机会。