LIVING PROSTHETIC SKIN

这项拟议的研究的目标是开发一种活的替代品 可用于治疗严重烧伤和皮肤脱落的皮肤。 最近在其中一个实验室里开发出了新的方法 在人类特定的培养基中方便快速地生长的协查人员 表皮角质形成细胞，并用于控制角质形成细胞的分化 文化。此外，一种可缓慢生物降解的合成真皮薄膜 由交联胶原蛋白和糖胺聚糖组成的化合物最近被 用于治疗严重烧伤的文献中所描述的。整体而言 研究计划是建立表皮角质形成细胞的培养 小皮肤活检，在培养中扩增细胞，在膜上涂上 培养角质形成细胞，诱导角质形成细胞分层，并 末端分化外层，然后用活的 为移植而产生的人造皮肤。文化将会是 在所有阶段进行仔细监测，以核实是否没有恶性 转化和不良的抗原性变化。跨学科的 由基础科学家和医生组成的研究团队已与 所有需要的细胞培养，皮肤病，生物化学， 这个项目需要烧伤的肿瘤学和临床管理。我们 预计此过程将允许来自少量 烧伤患者未烧伤皮肤数量迅速扩张并习惯于 准备一个移植物，然后可以永久地放在一个大的烧伤的上面 拒绝风险最小的区域。这样的程序应该大大 减少大面积烧伤患者的总治疗时间 减少移植所需的供体组织的数量，以及 增加救治严重烧伤病人的机会。

The objective of the proposed research is to develop a living replacement of skin that can be used in the treatment of major burns and skin losses. New methods have recently been developed in the laboratory of one of the co-investigators for convenient and rapid growth in defined medium of human epidermal keratinocytes, and for control of keratinocyte differentiation in culture. In addition, a slowly biodegradable synthetic dermal membrane composed of crosslinked collagen and glycosaminoglycans has recently been described in the literature for use in treating major burns. The overall research plan is to establish cultures of epidermal keratinocytes from a small skin biopsy, expand the cells in culture, coat the membrane with cultured keratinocytes, induce stratification of the keratinocytes and terminal differentiation of the outer layer, and then use the living artificial skin that has been generated for grafting. The cultures will be carefully monitored at all stages to verify absence of malignant transformation and undesirable antigenic changes. An interdisciplinary research team of basic scientists and physicians has been assembled with all of the needed expertise in cell culture, dermatology, biochemistry, oncology and clinical management of burns needed for this project. We anticipate that this procedure will allow cells from a small amount of a burn patient's unburned skin to be expanded rapidly in number and used to prepare a graft that can then be placed permanently over a large burned area with minimum risk of rejection. Such a procedure should greatly reduce total treatment time for patients with burns over a large area of the body, reduce the amount of donor tissue required for grafting, and increase the chances of saving very severely burned patients.