LIVING PROSTHETIC SKIN

拟议研究的目标是开发一种活的替代品， 可以用于治疗严重烧伤和皮肤缺损的皮肤。 最近在一个实验室里开发了新的方法， 共同研究者在人类确定的培养基中方便和快速生长 表皮角质形成细胞，并用于控制角质形成细胞分化， 文化 此外，一种缓慢生物降解的合成真皮膜 由交联的胶原蛋白和糖胺聚糖组成， 在文献中描述了用于治疗严重烧伤。 整体 研究计划是建立表皮角质形成细胞的培养物， 小的皮肤活检，在培养中扩大细胞，用 培养的角质形成细胞，诱导角质形成细胞分层， 终末分化的外层，然后使用生活 为移植而生成的人造皮肤。 将对培养物进行 在所有阶段进行仔细监测，以验证是否存在恶性肿瘤。 转化和不期望的抗原性变化。 一个跨学科 由基础科学家和医生组成的研究团队已经组建， 细胞培养皮肤病学生物化学 本项目所需的烧伤肿瘤学和临床管理。 我们 预计这一过程将允许细胞从少量的 烧伤病人未烧伤的皮肤在数量上迅速膨胀， 准备一个移植物，然后可以永久地放置在一个大的烧伤 拒绝风险最小的区域。 这样的程序应该大大 减少大面积烧伤患者的总治疗时间， 身体，减少移植所需的供体组织的量，以及 增加抢救严重烧伤病人的机会。

The objective of the proposed research is to develop a living replacement of skin that can be used in the treatment of major burns and skin losses. New methods have recently been developed in the laboratory of one of the co-investigators for convenient and rapid growth in defined medium of human epidermal keratinocytes, and for control of keratinocyte differentiation in culture. In addition, a slowly biodegradable synthetic dermal membrane composed of crosslinked collagen and glycosaminoglycans has recently been described in the literature for use in treating major burns. The overall research plan is to establish cultures of epidermal keratinocytes from a small skin biopsy, expand the cells in culture, coat the membrane with cultured keratinocytes, induce stratification of the keratinocytes and terminal differentiation of the outer layer, and then use the living artificial skin that has been generated for grafting. The cultures will be carefully monitored at all stages to verify absence of malignant transformation and undesirable antigenic changes. An interdisciplinary research team of basic scientists and physicians has been assembled with all of the needed expertise in cell culture, dermatology, biochemistry, oncology and clinical management of burns needed for this project. We anticipate that this procedure will allow cells from a small amount of a burn patient's unburned skin to be expanded rapidly in number and used to prepare a graft that can then be placed permanently over a large burned area with minimum risk of rejection. Such a procedure should greatly reduce total treatment time for patients with burns over a large area of the body, reduce the amount of donor tissue required for grafting, and increase the chances of saving very severely burned patients.