INTERPHASE SOLUBILITY AND CHRMOATOGRAPHIC RETENTION

建议的工作，是一个结合实验和理论的努力， 两组，旨在为预测分子生物学提供基础， 在物质的“界面”内的溶解度，特别是静止的 反相液相色谱（RPLC）的烃相，以及 包括两亲性聚集体如脂双层膜，囊泡， 和胶束。 我们以前已经发展了统计力学理论来预测 膜和胶束中的分子组织。 预测良好 与x射线衍射、小角中子衍射结果一致 散射、2 H、13 C、19 F NMR和状态方程测量。 的 主要结论是链状分子界面不是体相 但与界面相非常相似。 体相 热力学的依数性和分配系数， 不适用于这些系统;在这里，我们的目标是开发更合适的 溶解度的热力学和统计力学理论 间期 我们的实验工作一直致力于理论和 色谱系统的应用表征。 在这里，我们的目标是测试 严格的理论模型，通过系统的研究溶质的大小和 接枝物的化学性质、链长和表面密度 相间链，并通过建立有效的参考状态。 这项工作应直接关系到：i）保留机制的RPLC， 最广泛使用和发展最快的色谱分析方法 分离，以及ii）依数性的基础物理化学 脂质双层、囊泡和生物膜的性质，并且可以 为了解麻醉剂的分子机制提供了基础 药物作用。

The proposed work, to be a combined experimental and theoretical effort of two groups, aims to provide a foundation for predicting molecular solubilities within "interphases" of matter, especially the stationary hydrocarbon phases of leversed Phase Liquid Chromatography, (RPLC), and including amphiphilic aggregates such as lipid bilayer membranes, vesicles, and micelles. We have previously developed statistical mechanical theory to predict molecular organization in membranes and micelles. Predictions are in good agreement with results of x-ray diffraction, small angle neutron scattering, 2H, 13C, 19F NMR, and equation of state measurements. The principal conclusion is that chain molecule interphases are not bulk phases of matter, but bear close resemblance to interfacial phases. Bulk phase thermodynamics of colligative properties and of partition coefficients is not applicable to these systems; here we aim to develop more appropriate thermodynamic and statistical mechanical theory for solubilities in interphases. Our experimental efforts have been directed toward both theoretical and applied characterization of chromatographic systems. Here, we aim to test rigorously theoretical models through systematic study of solute size and chemical character, chain length and surface density of the grafted interphase chains, and through the establishment of valid reference states. This work should have direct bearing on: i) Retention mechanisms of RPLC, the most widely used and rapidly growing method of chromatographic separation, and ii) Fundamental physical chemistry of colligative properties of lipid bilayers, vesicle, and biological membranes, and may provide a foundation for understanding molecular mechanisms of anesthetic drug action.