REGULATION OF ETHYLENE-INDUCED GENE EXPRESSION

研究基因表达调控的一个很好的系统 植物的高级发育是乙烯激素诱导的果实成熟 番茄(西红柿)加工。在这份提案中， 乙烯诱导的基因表达程序将被分析，基因 其表达显著增加的基因将被分离出来，而DNA 控制其表达的序列将被识别出来。为此， 将构建含有推测的5‘上游的嵌合基因 与章鱼碱合成酶编码序列连接的调控序列，a 在内源番茄中很容易鉴定出其mRNA的基因 MRNAs。嵌合基因将被插入到番茄基因组植物中 从转化的细胞系中再生，并表达嵌合体 对再生植株果实中的基因进行了分析。这些实验将 提供有关基因表达如何调控的新的基本信息 在高等生物的发育过程中。

An excellent system for studying the regulation of gene expression during higher plant development is the ethylene hormone induced fruit ripening process in tomatoes (Lycopersicon esculentum). In this proposal the program of gene expression induced by ethylene will be analyzed, genes whose expression significantly increases will be isolated, and the DNA sequences that control their expression will be identified. To this end, chimeric genes will be constructed containing putative 5' upstream regulatory sequences ligated to coding sequences of octopine synthase, a gene whose mRNA can readily be identified amongst the endogenous tomato mRNAs. The chimeric genes will be inserted into the tomato genome, plants regenerated from transformed cell lines, and the expression of the chimeric genes assayed in fruit from the regenerated plants. These experiments will provide new and basic information about how gene expression is regulated during development in higher organisms.