REGULATION OF GENE EXPRESSION BY ETHYLENE

植物激素乙烯对植物的生长有着深远的影响。 高等植物组织器官的生长和分化。 为了阐明乙烯作用的机制， 调节乙烯诱导基因表达的细胞事件 将被定义。 具体而言：1）遗传上 乙烯的反应将被确定。 这将是完成 通过检测乙烯编码的mRNA的积累， 使用原位杂交在特定细胞中的诱导基因 技术. 2)DNA调控元件控制着 将定义乙烯诱导的基因表达。 这将是 通过在体外改变克隆的DNA序列， 乙烯诱导基因 植物会被转化为 诱变基因和损伤对基因表达的影响 将被评估。 3)与细胞因子相互作用， 将分离克隆的乙烯诱导基因。 这将是 通过分离核蛋白来完成， 与DNA调控序列相关联 从这些 实验将提供重要的线索， 乙烯诱导的基因表达及其作用机制 植物发育过程中激素作用。

The plant hormone ethylene has a profound influence on the growth and differentiation of tissues and organs in higher plants. To elucidate the mechanism of ethylene action, the molecular and cellular events which regulate ethylene-inducible gene expression will be defined. Specifically: 1) The cell types that genetically respond to ethylene will be determined. This will be accomplished by detecting the accumulation of mRNAs encoded by ethylene- inducible genes in specific cells using in situ hybridization techniques. 2) The DNA regulatory elements that control ethylene-inducible gene expression will be defined. This will be accomplished by altering in vitro the DNA sequence of cloned ethylene-inducible genes. Plants will be transformed with the mutagenized genes and the effect of the lesion on gene expression will be assessed. 3) The cellular factors that interact with the cloned ethylene-inducible genes will be isolated. This will be accomplished by isolating nuclear proteins that specifically bind to the DNA regulatory sequences. Results from these experiments will provide important clues about the regulation of gene expression by ethylene and the mechanism of ethylene hormone action during plant development.