REGULATION OF ETHYLENE-INDUCED GENE EXPRESSION

一个很好的研究基因表达调控的系统， 高等植物发育是乙烯激素诱导的果实成熟 番茄（Lycopersicon esculentum） 在这项提案中， 分析乙烯诱导的基因表达程序， 其表达显著增加的基因将被分离， 将鉴定控制其表达的序列。 为此目的， 将构建含有推定5'上游的嵌合基因 连接到章鱼碱合酶的编码序列的调节序列， 其mRNA可以容易地在内源番茄中鉴定的基因 mRNA。 嵌合基因将被插入番茄基因组， 从转化的细胞系再生，并且嵌合的 在来自再生植物的果实中测定的基因。 这些实验将 提供有关基因表达如何调节的新的基本信息 在高等生物的发育过程中。

An excellent system for studying the regulation of gene expression during higher plant development is the ethylene hormone induced fruit ripening process in tomatoes (Lycopersicon esculentum). In this proposal the program of gene expression induced by ethylene will be analyzed, genes whose expression significantly increases will be isolated, and the DNA sequences that control their expression will be identified. To this end, chimeric genes will be constructed containing putative 5' upstream regulatory sequences ligated to coding sequences of octopine synthase, a gene whose mRNA can readily be identified amongst the endogenous tomato mRNAs. The chimeric genes will be inserted into the tomato genome, plants regenerated from transformed cell lines, and the expression of the chimeric genes assayed in fruit from the regenerated plants. These experiments will provide new and basic information about how gene expression is regulated during development in higher organisms.