Confocal spectroscopy for cell 3D microthermometry
用于细胞 3D 显微测温的共焦光谱
基本信息
- 批准号:BB/X003841/1
- 负责人:
- 金额:$ 23.17万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2023
- 资助国家:英国
- 起止时间:2023 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
One in two people will be diagnosed with cancer during their lifetime, presenting a significant challenge to the UK goal of "staying healthy for longer". For some cancers, therapeutic innovations have increased survival, but for other cancers, such as brain cancer, outcomes have changed little in 20 years. A topic of increasing interest in the cancer research community is the critical role of metabolism in cancer cell behaviour. The Warburg effect, a metabolic switch from oxidative to glycolytic metabolism in cancer cells, has been documented for over decades, but much remains unknown about the nature and significance of cancer cell metabolism. The intrinsic pyrogenic substances secreted by tumour cells induce distinct hyperthermia in the temperature range of 37 to 42 C. Simultaneously, different parts of the cell can be at different temperatures, with mitochondria more than 10 C above basal temperature.We need to investigate fundamental unknowns about cancer cell metabolism, its role in cancer growth and the potential of targeting more metabolically active regions within cancer for therapy. Significantly, there is increasing awareness that this needs to be done in the context of intact cancer tissue, where the cancer cell interactions with the cellular microenvironment can be observed. Cancer cell-microenvironment interactions influence cancer cell biology and are not effectively modelled using in vitro cancer cell cultures. Crucially, then, cancer cell metabolism must be interrogated in tissue slice culture, and ultimately in rodent models, for which we need innovative technologies as proposed here.For this, it is necessary to have an imaging technique capable of working in three dimensions in thick tissue, and able to provide the temperature distributions in the cancer environment. This can be achieved by using luminescent nanoparticles as probes. Such nanoparticles can be 500 times smaller than a red blood cell, and when they are excited with light of a wavelength ("colour"), they will re-emit light in a different wavelength. The analysis of this re-emitted light can provide information about the temperature of its environment. Importantly, certain wavelengths can propagate longer in tissue without being attenuated, which can be used for obtaining information from inner areas. This will enable the 3D reconstruction of the map of temperatures.
每两个人中就有一个会在他们的一生中被诊断出患有癌症,这对英国“保持健康更长时间”的目标提出了重大挑战。对于某些癌症,治疗创新提高了患者的存活率,但对于其他癌症,如脑癌,20年来治疗结果几乎没有变化。代谢在癌细胞行为中的关键作用是癌症研究界日益关注的一个话题。沃伯格效应,癌细胞中从氧化代谢到糖酵解代谢的代谢转换,已经被记录了几十年,但关于癌细胞代谢的性质和意义仍然未知。肿瘤细胞分泌的固有热原物质在37 ~ 42℃的温度范围内诱导不同的高热。同时,细胞的不同部位可以处于不同的温度,线粒体比基础温度高10℃以上。我们需要调查关于癌细胞代谢的基本未知,它在癌症生长中的作用,以及在癌症中靶向代谢更活跃的区域进行治疗的潜力。值得注意的是,人们越来越意识到这需要在完整的癌症组织中进行,在那里可以观察到癌细胞与细胞微环境的相互作用。癌细胞-微环境相互作用影响癌细胞生物学,并且不能有效地使用体外癌细胞培养模型。因此,至关重要的是,癌细胞的代谢必须在组织切片培养中进行研究,并最终在啮齿动物模型中进行研究,为此我们需要这里提出的创新技术。为此,有必要拥有一种能够在厚组织中三维工作的成像技术,并能够提供癌症环境中的温度分布。这可以通过使用发光纳米粒子作为探针来实现。这种纳米粒子可以比红细胞小500倍,当它们被一个波长的光(“颜色”)激发时,它们会重新发出不同波长的光。对这种重新发射的光的分析可以提供有关其环境温度的信息。重要的是,某些波长可以在组织中传播更长时间而不被衰减,这可以用于从内部区域获取信息。这将使温度图的三维重建成为可能。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Proteomic and yeast 2-hybrid screens to identify PTEN binding partners
- DOI:10.1016/j.jbior.2023.100989
- 发表时间:2024-02-21
- 期刊:
- 影响因子:0
- 作者:Tibarewal,Priyanka;Spinelli,Laura;Leslie,Nick R.
- 通讯作者:Leslie,Nick R.
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Jose Marques-Hueso其他文献
Jose Marques-Hueso的其他文献
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{{ truncateString('Jose Marques-Hueso', 18)}}的其他基金
Multimaterial Stereolithography by Crosslinking through Luminescence Excitation
通过发光激发交联的多材料立体光刻
- 批准号:
EP/T013680/1 - 财政年份:2020
- 资助金额:
$ 23.17万 - 项目类别:
Research Grant
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