课题基金 / 基金详情

DYNAMICS OF ACTIN FILAMENTS IN NERVE GROWTH

DYNAMICS OF ACTIN FILAMENTS IN NERVE GROWTH
肌动蛋白丝在神经生长中的动力学
批准号:
3287279
负责人:
JAMES R BAMBURG
金额:
$13.76万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-12-01 至 1989-11-30

项目摘要

项目成果

JAMES R BAMBURG的其他基金

相似基金

相关文献

中文摘要
翻译
利用G-肌动蛋白抑制水解酶的能力来测定G-肌动蛋白 脱氧核糖核酸酶的活性,我们从大脑中分离出一种蛋白质,它具有 促进F-肌动蛋白在溶液中的解聚。这种蛋白质现在将是 进一步表征了其相对分子质量、等电点、氨基酸 F-肌动蛋白的组成及其单独与F-肌动蛋白相互作用的能力 原肌球蛋白等相关蛋白。 一种改进的DNA酶抑制试验已经被开发出来,它允许 G-肌动蛋白在F-肌动蛋白存在下的测定 两个肌动蛋白池之间发生交换。这项检测将应用于 同步化CHO细胞中G和F-肌动蛋白浓度的测定 细胞周期。
英文摘要
Using an assay for G-actin based on its ability to inhibit the hydrolytic activity of DNAase, we have isolated a protein from brain which actively promotes the depolymerization of F-actin in solution. This protein will now be further characterized as to its molecular weight, isoelectric point, amino acid composition and its ability to interact with F-actin alone and in the presence of associated proteins such as tropomyosin. A modification of the DNAase inhibition assay has been developed which permits the assay of G-actin in the presence of F-actin under conditions where no exchange between the two actin pools occurs. This assay will be applied to the measurement of G and F-actin concentrations in synchronized CHO cells during the cell cycle.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Role of cofilin pathology in mouse models of cognitive impairment
  • 批准号:
    8664331
  • 项目类别:
  • 资助金额:
    $18.59万
  • 财政年份:
    2013
  • 负责人:
    JAMES R BAMBURG
  • 依托单位:
Role of cofilin pathology in mouse models of cognitive impairment
  • 批准号:
    8486049
  • 项目类别:
  • 资助金额:
    $22.3万
  • 财政年份:
    2013
  • 负责人:
    JAMES R BAMBURG
  • 依托单位:
ISOLATION AND CHARACTERIZATION OF CYTOPLASMIC COFILIN-ACTIN RODS
  • 批准号:
    8171304
  • 项目类别:
  • 资助金额:
    $0.08万
  • 财政年份:
    2010
  • 负责人:
    JAMES R BAMBURG
  • 依托单位:
Training in Synaptic Neurobiology
  • 批准号:
    6768597
  • 项目类别:
  • 资助金额:
    $25.73万
  • 财政年份:
    2002
  • 负责人:
    JAMES R BAMBURG
  • 依托单位:
海外基金