DYNAMICS OF ACTIN FILAMENTS IN NERVE GROWTH
DYNAMICS OF ACTIN FILAMENTS IN NERVE GROWTH
批准号:
3287279
负责人:
JAMES R BAMBURG
金额:
$13.76万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-12-01 至 1989-11-30
关键词:
adenosinetriphosphatase affinity chromatography autoradiography brain brain metabolism cell growth regulation cell population study circular dichroism conformation density gradient ultracentrifugation electrofocusing electron microscopy gel electrophoresis glia ions local anesthetics messenger RNA neuropharmacology neurotrophic factors stoichiometry tissue /cell culture ultraviolet spectrometry wheat
中文摘要
利用G-肌动蛋白抑制水解酶的能力来测定G-肌动蛋白
脱氧核糖核酸酶的活性,我们从大脑中分离出一种蛋白质,它具有
促进F-肌动蛋白在溶液中的解聚。这种蛋白质现在将是
进一步表征了其相对分子质量、等电点、氨基酸
F-肌动蛋白的组成及其单独与F-肌动蛋白相互作用的能力
原肌球蛋白等相关蛋白。
一种改进的DNA酶抑制试验已经被开发出来,它允许
G-肌动蛋白在F-肌动蛋白存在下的测定
两个肌动蛋白池之间发生交换。这项检测将应用于
同步化CHO细胞中G和F-肌动蛋白浓度的测定
细胞周期。
英文摘要
Using an assay for G-actin based on its ability to inhibit the hydrolytic
activity of DNAase, we have isolated a protein from brain which actively
promotes the depolymerization of F-actin in solution. This protein will now be
further characterized as to its molecular weight, isoelectric point, amino acid
composition and its ability to interact with F-actin alone and in the presence
of associated proteins such as tropomyosin.
A modification of the DNAase inhibition assay has been developed which permits
the assay of G-actin in the presence of F-actin under conditions where no
exchange between the two actin pools occurs. This assay will be applied to the
measurement of G and F-actin concentrations in synchronized CHO cells during the
cell cycle.
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会议论文
Role of cofilin pathology in mouse models of cognitive impairment
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批准号:8664331
-
项目类别:
-
资助金额:$18.59万
-
财政年份:2013
-
负责人:JAMES R BAMBURG
-
依托单位:
Role of cofilin pathology in mouse models of cognitive impairment
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批准号:8486049
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项目类别:
-
资助金额:$22.3万
-
财政年份:2013
-
负责人:JAMES R BAMBURG
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依托单位:
ISOLATION AND CHARACTERIZATION OF CYTOPLASMIC COFILIN-ACTIN RODS
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批准号:8171304
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项目类别:
-
资助金额:$0.08万
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财政年份:2010
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负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
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批准号:6768597
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项目类别:
-
资助金额:$25.73万
-
财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
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批准号:6919974
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项目类别:
-
资助金额:$25.81万
-
财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
-
批准号:7096532
-
项目类别:
-
资助金额:$19.29万
-
财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
-
批准号:6452374
-
项目类别:
-
资助金额:$24.71万
-
财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
-
批准号:6604211
-
项目类别:
-
资助金额:$25.73万
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财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
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批准号:6617076
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项目类别:
-
资助金额:$1.49万
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财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
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批准号:6685913
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项目类别:
-
资助金额:$24.11万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
-
批准号:6826802
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项目类别:
-
资助金额:$24.11万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/cofilin-actin rods in neurodegenerative diseases
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批准号:7413273
-
项目类别:
-
资助金额:$32.12万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
-
批准号:6620582
-
项目类别:
-
资助金额:$24.11万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/cofilin-actin rods in neurodegenerative diseases
-
批准号:7591023
-
项目类别:
-
资助金额:$32.12万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
-
批准号:6419165
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项目类别:
-
资助金额:$26.87万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/cofilin-actin rods in neurodegenerative diseases
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批准号:7145732
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项目类别:
-
资助金额:$31.82万
-
财政年份:2000
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/cofilin-actin rods in neurodegenerative diseases
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批准号:7236176
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项目类别:
-
资助金额:$32.08万
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财政年份:2000
-
负责人:JAMES R BAMBURG
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依托单位:
ADF/COFILIN IN EARLY VERTEBRATE DEVELOPMENT
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批准号:2023273
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项目类别:
-
资助金额:$17.07万
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财政年份:1997
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负责人:JAMES R BAMBURG
-
依托单位:
ADF/COFILIN IN EARLY VERTEBRATE DEVELOPMENT
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批准号:2900873
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项目类别:
-
资助金额:$20.64万
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财政年份:1997
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/COFILIN IN EARLY VERTEBRATE DEVELOPMENT
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批准号:2685100
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项目类别:
-
资助金额:$19.55万
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财政年份:1997
-
负责人:JAMES R BAMBURG
-
依托单位:
海外基金