REGULATION OF HISTIDINE BIOSYNTHESIS IN YEAST
REGULATION OF HISTIDINE BIOSYNTHESIS IN YEAST
批准号:
3287064
负责人:
GERALD R FINK
金额:
$31.55万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-07-01 至 1985-11-30
关键词:
Saccharomyces aminoacid biosynthesis cell growth regulation chromosome aberrations chromosome disorders enzyme substrate complex eukaryote fungal genetics gene expression genetic manipulation genetic mapping genetic recombination genetic transcription genetic translation histidine molecular cloning nucleic acid sequence regulatory gene structural genes temperature sensitive mutant transfer RNA
中文摘要
我们的目标是了解酵母中基因活动的调控
英文摘要
Our goal is to understand the regulation of gene activity in the yeast
Saccharomyces cerevisiae. The studies will focus on the nucleotide sequences
which regulate transcription and translation of the histidine biosynthetic
enzymes. Mutations in these sequences will be obtained by in vitro and in vovo
mutagenesis. The mutations derived in vivo will be cloned by recombinant DNA
procedures and then sequenced; those constructed in vitro will be inserted back
into the genome for study by transformation.
One of the sequences responsible for regulating genes in yeast is the
transposable element, TY1. This element causes loss of function by insertion
into the 5' non-coding region prior to the first ATG in the gene. We will study
the insertion and excision of this element genetically and biochemically.
Genetically, we will study the chromosome aberrations caused by the element as
well as characterize mutations which enhance or diminish its insertion and
excision. We will characterize the join points between the target gene and the
element as well as the specificity of the recombination event by nucleotide
sequence analysis. The regulatory effort of the insertion element on the
transcription capabilities of the adajcent structural gene will be studied by
Northern analysis.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
MOLECULAR BIOLOGY OF INFECTIONS DISEASE
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批准号:2058126
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项目类别:
-
资助金额:$22.63万
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财政年份:1988
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负责人:GERALD R FINK
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依托单位:
BIOMEDICAL RESEARCH SUPPORT GRANT
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批准号:3517281
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项目类别:
-
资助金额:$4.93万
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财政年份:1985
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负责人:GERALD R FINK
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依托单位:
REGULATION OF HISTIDINE BIOSYNTHESIS IN YEAST
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批准号:2177702
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项目类别:
-
资助金额:$43.31万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
BIOLOGICAL FUSIONS--CONJUGATION IN YEAST
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批准号:2180227
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项目类别:
-
资助金额:$32.83万
-
财政年份:1984
-
负责人:GERALD R FINK
-
依托单位:
REGULATION OF HISTIDINE BIOSYNTHESIS IN YEAST
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批准号:2177703
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项目类别:
-
资助金额:$39.84万
-
财政年份:1984
-
负责人:GERALD R FINK
-
依托单位:
BIOLOGICAL FUSIONS--CONJUGATION IN YEAST
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批准号:2022230
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项目类别:
-
资助金额:$34.88万
-
财政年份:1984
-
负责人:GERALD R FINK
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依托单位:
BIOLOGICAL FUSIONS--CONJUGATION IN YEAST
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批准号:2900683
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项目类别:
-
资助金额:$36.97万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
GENETIC CONTROL OF NUTRITIONAL STARVATION IN YEAST
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批准号:6046024
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项目类别:
-
资助金额:$49.22万
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财政年份:1984
-
负责人:GERALD R FINK
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依托单位:
REGULATION OF HISTIDINE BIOSYNTHESIS IN YEAST
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批准号:2608836
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项目类别:
-
资助金额:$42.0万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
REGULATION OF HISTIDINE BIOSYNTHESIS IN YEAST
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批准号:3484814
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项目类别:
-
资助金额:$38.16万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
GENETIC CONTROL OF NUTRITIONAL STARVATION IN YEAST
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批准号:6625046
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项目类别:
-
资助金额:$46.93万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
The Role of Genetic Variation in the Fungal/Host Interaction
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批准号:7893581
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项目类别:
-
资助金额:$82.98万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
Biological Fusions Conjugations in Yeast
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批准号:6919442
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项目类别:
-
资助金额:$70.33万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
Biological Fusions Conjugations in Yeast
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批准号:7217433
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项目类别:
-
资助金额:$70.63万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
Genetic Control of Nutrition Starvation in Yeast
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批准号:7152506
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项目类别:
-
资助金额:$62.94万
-
财政年份:1984
-
负责人:GERALD R FINK
-
依托单位:
Genetic Control of Nutrition Starvation in Yeast
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批准号:6986202
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项目类别:
-
资助金额:$63.2万
-
财政年份:1984
-
负责人:GERALD R FINK
-
依托单位:
Genetic Control of Nutrition Starvation in Yeast
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批准号:9081594
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项目类别:
-
资助金额:$55.28万
-
财政年份:1984
-
负责人:GERALD R FINK
-
依托单位:
REGULATION OF HISTIDINE BIOSYNTHESIS IN YEAST
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批准号:3484813
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项目类别:
-
资助金额:$35.06万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
Genetic Control of Nutrition Starvation in Yeast
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批准号:6727879
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项目类别:
-
资助金额:$62.04万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
GENETIC CONTROL OF NUTRITIONAL STARVATION IN YEAST
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批准号:6476458
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项目类别:
-
资助金额:$45.94万
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财政年份:1984
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负责人:GERALD R FINK
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依托单位:
海外基金