GENE EXPRESSION DURING PSEUDOMONAS TOMATO INFECTION

研究了番茄与其寄主植物的互作关系。 病原菌，假单胞菌pv.番茄，提供一个很好的系统， 研究植物基因在寄主-病原体相互作用过程中的表达。 在抗病植物中表达的基因（“防御”基因） 在细菌感染期间病原体将被分离，并且它们的结构 并确定基因表达程序。 这些序列 重要的“防御”基因表达的调节将被确定 通过缺失突变和基因融合分析。 完整基因和修饰基因 将被引入到对疾病敏感的番茄基因组中;转化 植物将被再生并分析其结构，组织， 和“防御”基因的表达。 此外，转化植物将 测试抗病表型的表达。 这些 实验将提供关于植物基因如何 在高等真核生物的生物胁迫过程中调节表达。

The interactions between the tomato plant (Lycopersicon esculentum) and its pathogen, Pseudomonas syringae pv. tomato, provide an excellent system for the study of plant gene expression during host-pathogen interactions. Genes which are expressed in a disease-resistant plant ("defense" genes) during bacterial pathogen infection will be isolated, and their structure and gene expression programs will be determined. The sequences which are important in the regulation of "defense" gene expression will be identified by deletion mutant and gene fusion analysis. Intact and modified genes will be introduced into the disease-sensitive tomato genome; transformed plants will be regenerated and analyzed for the structure, organization, and expression of the "defense" gene. In addition, transformed plants will be tested for the expression of the disease-resistance phenotype. These experiments will provide new and basic information about how plant gene expression is regulated during a biological stress in a higher eukaryotic.