GENE EXPRESSION DURING PSEUDOMONAS TOMATO INFECTION

番茄假单胞菌感染期间的基因表达

• 批准号: 3289374
• 负责人: LINDA L WALLING
• 金额: $ 7.47万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3289374
• 关键词: Pseudomonas; R factors; bacterial cytopathogenic effect; bacterial genetics; chromosome deletion; complementary DNA; endonuclease; gene expression; gene interaction; genetic manipulation; genetic mapping; genetic regulation; genetic transcription; genetic translation; molecular cloning; molecular genetics; nucleic acid sequence; nucleic acid structure; plant diseases; plant genetics; stress; tomato

The interactions between the tomato plant (Lycopersicon esculentum) and its pathogen, Pseudomonas syringae pv. tomato, provide an excellent system for the study of plant gene expression during host-pathogen interactions. Genes which are expressed in a disease-resistant plant ("defense" genes) during bacterial pathogen infection will be isolated, and their structure and gene expression programs will be determined. The sequences which are important in the regulation of "defense" gene expression will be identified by deletion mutant and gene fusion analysis. Intact and modified genes will be introduced into the disease-sensitive tomato genome; transformed plants will be regenerated and analyzed for the structure, organization, and expression of the "defense" gene. In addition, transformed plants will be tested for the expression of the disease-resistance phenotype. These experiments will provide new and basic information about how plant gene expression is regulated during a biological stress in a higher eukaryotic.

番茄植株（Lycopersicon esculentum）与植物间的相互作用