GENE EXPRESSION DURING PSEUDOMONAS TOMATO INFECTION

番茄假单胞菌感染期间的基因表达

• 批准号: 3289373
• 负责人: LINDA L WALLING
• 金额: $ 7.9万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3289373
• 关键词: Pseudomonas; R factors; bacterial cytopathogenic effect; bacterial genetics; chromosome deletion; complementary DNA; endonuclease; gene expression; gene interaction; genetic manipulation; genetic mapping; genetic regulation; genetic transcription; genetic translation; molecular cloning; molecular genetics; nucleic acid sequence; nucleic acid structure; plant diseases; plant genetics; stress; tomato

The interactions between the tomato plant (Lycopersicon esculentum) and its pathogen, Pseudomonas syringae pv. tomato, provide an excellent system for the study of plant gene expression during host-pathogen interactions. Genes which are expressed in a disease-resistant plant ("defense" genes) during bacterial pathogen infection will be isolated, and their structure and gene expression programs will be determined. The sequences which are important in the regulation of "defense" gene expression will be identified by deletion mutant and gene fusion analysis. Intact and modified genes will be introduced into the disease-sensitive tomato genome; transformed plants will be regenerated and analyzed for the structure, organization, and expression of the "defense" gene. In addition, transformed plants will be tested for the expression of the disease-resistance phenotype. These experiments will provide new and basic information about how plant gene expression is regulated during a biological stress in a higher eukaryotic.

番茄植株 (Lycopersicon esculentum) 及其植物之间的相互作用 病原体，丁香假单胞菌 pv。番茄，提供了一个优秀的系统 研究宿主与病原体相互作用期间植物基因表达。 在抗病植物中表达的基因（“防御”基因） 细菌感染病原体时会被分离出来，其结构 并将确定基因表达程序。 的序列是 将鉴定在“防御”基因表达调节中的重要作用 通过缺失突变体和基因融合分析。 完整和修饰的基因 将被引入对疾病敏感的番茄基因组中；转变的 植物将被再生并分析其结构、组织、 以及“防御”基因的表达。 此外，转化植物将 检测抗病表型的表达。 这些 实验将提供有关植物基因如何运作的新的基本信息 在高等真核生物中，表达在生物应激期间受到调节。