GENE EXPRESSION DURING PSEUDOMONAS TOMATO INFECTION

番茄与番茄的相互作用 病原菌为紫丁香假单胞菌。番茄，提供了一种极好的系统 寄主-病原菌相互作用过程中植物基因表达的研究。 在抗病植物中表达的基因(“防御”基因) 在细菌感染期间将病原体分离出来，并将其结构 基因表达计划将被确定。这些序列是 在“防御”基因表达调控中的重要作用将被确定 通过缺失突变和基因融合分析。完整和修饰的基因 将被导入对疾病敏感的番茄基因组；转化 植物将被再生并分析其结构、组织、 以及“防御”基因的表达。此外，转化后的植物将 检测抗病表型的表达。这些 实验将提供新的和基本的信息关于植物基因如何 在高等真核生物中，表达在生物胁迫过程中受到调控。

The interactions between the tomato plant (Lycopersicon esculentum) and its pathogen, Pseudomonas syringae pv. tomato, provide an excellent system for the study of plant gene expression during host-pathogen interactions. Genes which are expressed in a disease-resistant plant ("defense" genes) during bacterial pathogen infection will be isolated, and their structure and gene expression programs will be determined. The sequences which are important in the regulation of "defense" gene expression will be identified by deletion mutant and gene fusion analysis. Intact and modified genes will be introduced into the disease-sensitive tomato genome; transformed plants will be regenerated and analyzed for the structure, organization, and expression of the "defense" gene. In addition, transformed plants will be tested for the expression of the disease-resistance phenotype. These experiments will provide new and basic information about how plant gene expression is regulated during a biological stress in a higher eukaryotic.