How does ASYNAPSIS 5 mediate Synaptonemal Complex formation and crossover control in plants?

ASYNAPSIS 5 如何介导植物联会复合体的形成和交叉控制？

• 批准号: BB/X006212/1
• 负责人: James Higgins
• 金额: $ 52.84万
• 依托单位: University of Leicester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FX006212%2F1
• 关键词: How; does; ASYNAPSIS; mediate; Synaptonemal

The majority of flowering plants (including crops) propagate through sexual reproduction where the male gamete (pollen) fertilises the female gamete (ovule). The gametes contain half the number of chromosomes of the parents that are restored to normal levels upon fertilisation. The halving of chromosomes occurs during meiosis, a specialised cell division characterised by homologous recombination. Homologous recombination shuffles segments of chromosomes, thus producing new combinations of genes in daughter cells. It is therefore a critical process for plant breeders in bringing advantageous traits (eg. yield) together for crop improvement. Efforts to increase the efficiency of gene shuffling in crops has been limited by internal constraints acting on this process; primarily the level of recombination and its tendency to be localized to certain chromosome regions and occur infrequently in others. An additional factor is many crop plants such as wheat, oil seed rape and potato are polyploids, that is they have multiple sets of chromosomes that are similar or identical. However, although such plants have adapted to solving this particular problem, they are still susceptible to meiotic failure that can yield in loss of fertility. In addition, this process is extremely sensitive to high temperature stress that is an increasing challenge due to global warming. Therefore, understanding how homologous recombination during meiosis is controlled at the molecular level will provide strategies for future-proofing plant breeding.We have already identified many of the genes essential for homologous recombination in plants, but our previous analysis predicted the existence of an essential unknown gene. We have now identified this gene in the model plant Arabidopsis thaliana and named it ASYNAPSIS5 (ASY5) based on its phenotype. Our preliminary data shows that it is a fundamental gene for promoting homologous recombination in flowering plants. Therefore, the aim of this proposal is to characterise the role of the ASY5 protein by molecular, cytological, genetical and biochemical techniques. We will first determine how ASY5 interacts with already known genes/proteins and then quantify how ASY5 mediates homologous recombination in asy5 mutants. This will be followed by an investigation into how ASY5 stabilises meiosis in the polyploid plant Arabidopsis lyrata as well as identifying which gene variants are more effective at promoting this process. Finally, we will determine the heat sensitivity of ASY5 in A. thaliana to discover the underlying molecular mechanism of why it fails at higher temperatures with the aim of identifying variants that are more heat tolerant.

大多数开花植物（包括农作物）通过有性生殖繁殖，其中雄配子（花粉）使雌配子（胚珠）受精。配子含有父母染色体数量的一半，受精后恢复到正常水平。染色体减半发生在减数分裂过程中，这是一种以同源重组为特征的特殊细胞分裂。同源重组使染色体的片段重组，从而在子细胞中产生新的基因组合。因此，这是植物育种者带来有利性状（例如）的关键过程。#21453;，共同提高农作物产量。提高作物基因改组效率的努力受到了作用于这一过程的内部约束的限制;主要是重组水平及其局限于某些染色体区域而很少在其他区域发生的趋势。另一个因素是许多作物植物如小麦、油菜和马铃薯是多倍体，也就是说它们具有多套相似或相同的染色体。然而，尽管这样的植物已经适应于解决这个特定的问题，但它们仍然容易发生减数分裂失败，从而导致生育力丧失。此外，这一过程对高温胁迫极其敏感，这是由于全球变暖而带来的日益严峻的挑战。因此，了解减数分裂过程中同源重组是如何在分子水平上控制的，将为未来的植物育种提供策略。我们已经确定了许多植物同源重组所必需的基因，但我们以前的分析预测了一个重要的未知基因的存在。我们现在已经在模式植物拟南芥中鉴定了该基因，并根据其表型将其命名为ASYNAPSIS 5（ASY 5）。我们的初步数据表明，它是一个基本基因，促进同源重组的显花植物。因此，本提案的目的是通过分子、细胞学、遗传学和生物化学技术来阐明ASY 5蛋白的作用。我们将首先确定ASY 5如何与已知的基因/蛋白质相互作用，然后量化ASY 5如何介导asy 5突变体中的同源重组。接下来将研究ASY 5如何稳定多倍体植物Arabidopsis lyrata的减数分裂，以及确定哪些基因变体在促进这一过程中更有效。最后，我们将测定ASY 5在A. thaliana发现其在高温下失败的潜在分子机制，目的是确定更耐热的变体。