DYNAMICS OF ACTIN FILAMENTS IN NERVE GROWTH

肌动蛋白丝在神经生长中的动力学

• 批准号: 3287275
• 负责人: JAMES R BAMBURG
• 金额: $ 12.99万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3287275
• 关键词: actins; adenosinetriphosphatase; affinity chromatography; autoradiography; brain; brain metabolism; cell growth regulation; cell population study; chick embryo; circular dichroism; conformation; density gradient ultracentrifugation; electrofocusing; electron microscopy; gel electrophoresis; glia; ions; laboratory rabbit; local anesthetics; messenger RNA; microfilaments; microtubules; neurogenesis; neuropharmacology; neurotrophic factors; protein biosynthesis; protein sequence; protein structure; stoichiometry; tissue /cell culture; ultraviolet spectrometry; wheat

Using an assay for G-actin based on its ability to inhibit the hydrolytic activity of DNAase, we have isolated a protein from brain which actively promotes the depolymerization of F-actin in solution. This protein will now be further characterized as to its molecular weight, isoelectric point, amino acid composition and its ability to interact with F-actin alone and in the presence of associated proteins such as tropomyosin. A modification of the DNAase inhibition assay has been developed which permits the assay of G-actin in the presence of F-actin under conditions where no exchange between the two actin pools occurs. This assay will be applied to the measurement of G and F-actin concentrations in synchronized CHO cells during the cell cycle.

利用基于g -肌动蛋白抑制水解能力的测定