Canada_IPAP: Amoebae with antimicrobial resistant endsymbionts (AWARE)

Canada_IPAP：具有抗菌素耐药性末端共生体的阿米巴原虫 (AWARE)

• 批准号: BB/X012824/1
• 负责人: Charles Knapp
• 金额: $ 18.84万
• 依托单位: University of Strathclyde
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FX012824%2F1
• 关键词: Canada_IPAP; Amoebae; antimicrobial; resistant; endsymbionts

Antimicrobial resistance represents bacteria's ability to withstand harmful chemicals; most popularly, their ability to resist antibiotics has been most concerning. These traits allow disease-causing pathogens (for example) to survive antibiotic therapy. Less commonly known are the impacts of "other" compounds (e.g., disinfectants) and other organisms in nature that may worsen the problem. In particular, quaternary ammonium compounds (QACs) are high-consumption chemicals used to make emulsifiers, fabric softeners, corrosion inhibitors and personal care products. They are also the most common active ingredients in sanitizing and disinfecting products; as such, they are a candidate for developing resistance. Therefore, we will examine their role in resistance development because of their extensive use in the food preparation industry. More importantly, The project examines the role of free-living amoebae (a protozoan found in waters and sediment) as a mechanism and vector in developing and spreading antimicrobial-resistant bacteria. Free-living amoebae harbour bacteria and essentially could be protected, to an extent, from toxic compounds in the environment. However, for some additional reason, they further enhance the potential for bacteria to become resistant. Some of the bacteria are fish pathogens. So their mechanism as a driving force for drug resistance must be investigated.This investigation will take place within a whole-lake exposure study at the Experimental Lakes Area (Ontario), which includes the impacts of multiple trophic levels within a lake--including Lake Trout. Comparisons of bacterial populations and their resistance traits in the water, sediment, amoebae and fish will be made as preliminary results towards understanding whether similarities in their distribution do occur and whether QAC disinfectants also contribute to the selection pressure. This is a partnership development grant with a unique opportunity to collaborate with other researchers to better understand the biological mechanisms of antimicrobial resistance in a real-life context and their risks in the aquaculture and fishing industries.

抗菌素耐药性代表细菌抵抗有害化学物质的能力；最普遍的是，他们抵抗抗生素的能力一直是最令人担忧的。这些特性使致病病原体(例如)在抗生素治疗中存活下来。不太为人所知的是“其他”化合物(如消毒剂)和自然界中可能使问题恶化的其他生物的影响。特别是，季铵化合物(QAC)是用于制造乳化剂、织物柔软剂、缓蚀剂和个人护理产品的高消耗化学品。它们也是消毒和消毒产品中最常见的活性成分；因此，它们是产生耐药性的候选物质。因此，我们将研究它们在抗药性发展中的作用，因为它们在食品加工行业中被广泛使用。更重要的是，该项目研究了自由生活的阿米巴(一种在水和沉积物中发现的原生动物)作为一种机制和媒介在发展和传播抗菌素耐药性细菌方面的作用。自由生活的阿米巴虫藏匿着细菌，基本上可以在一定程度上受到保护，免受环境中有毒化合物的伤害。然而，由于其他一些原因，它们进一步增强了细菌产生抗药性的可能性。其中一些细菌是鱼的病原体。因此，必须研究它们作为抗药性驱动力的机制。这项调查将在安大略省实验湖地区进行的一项全湖暴露研究中进行，该研究包括湖泊内多种营养水平的影响--包括鲑鱼。将对水、沉积物、阿米巴和鱼中的细菌种群及其抗性特征进行比较，作为初步结果，以了解它们的分布是否存在相似之处，以及QAC消毒剂是否也有助于选择压力。这是一项伙伴关系发展赠款，有一个独特的机会与其他研究人员合作，以更好地了解现实背景下抗菌剂耐药性的生物学机制及其在水产养殖和渔业中的风险。