GENE EXPRESSION IN CELL ORGANELLES

总的目标是阐明一些机制的控制和 眼虫叶绿体中依赖DNA的RNA转录调控 股薄肌 具体项目包括：（1）地点和DNA 叶绿体转录单位的序列分析和推测的调控基因 区域，（2）光照期间特定转录物变化的定量 诱导叶绿体发育，（3）在体外系统中表征 参与选择性RNA转录的组分。 在未来一年，我们计划 （1）继续进行部分DNA序列分析， 核糖体RNA转录单位和选定的mRNA编码区，包括 核酮糖-1，5-二磷酸羧化酶大亚基的基因，（2） 完成叶绿体RNA聚合酶亚基结构的研究（3） 通过溶液分析初级转录本和加工途径， 北方杂交分析一些特定的转录单位， DNA序列数据可用，（4）继续一般绘图程序， 裸藻叶绿体基因组的克隆和基因定位。

The overall objective is to elucidate some of the mechanisms for the control and regulation of DNA-dependent RNA transcription in chloroplasts of Euglena gracilis. Specific projects include the following: (1) Location and DNA sequence analysis of chloroplast transcription units and putative regulatory regions, (2) Quantitation of changes in specific transcripts during light induced chloroplast development, (3) Characterizing in an in vitro system the components involved in selective RNA transcription. In the coming year we plan the following projects: (1) Continue DNA sequence analysis of portions of the ribosomal RNA transcription units, and selected mRNA coding regions, including the gene for the large subunit of ribulose-1,5 -bis phosphate carboxylase, (2) Complete a study of the subunit structure of chloroplast RNA polymerase, (3) Analyze the primary transcripts and processing pathways via solution and Northern hybridization analysis for some specific transcription units for which DNA sequence data are available, (4) Continue a general program of mapping, cloning, and gene localization for the Euglena chloroplast genome.