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HORMONAL REGULATION OF ENDOMETRIAL DIFFERENTIATION

子宫内膜分化的激素调节

基本信息

批准号：
3312763
负责人：
P G SATYASWAROOP
金额：
$ 8万
依托单位：
PENNSYLVANIA STATE UNIV HERSHEY MED CTR
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-09-01 至 1986-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3312763
关键词：
cell differentiation cell population study endometrium estradiol genetic transcription hormone inhibitor hormone regulation /control mechanism human tissue menstrual cycle disorder organ culture phorbols progesterone radiotracer scintillation spectrometry steroid hormone tissue /cell culture

项目摘要

The concept of stem cell mediated differentiation that explains the process of tissue regenerations is proposed as a model for the cyclic renewal of human endometrium. Regulation of endometrial function by steroid hormones which forms the major part of the proposed scheme will be examined in the initial phase of the study. Accordingly, the cellular distribution of hormone-sensitive markers, such as receptors for estradiol and progesterone and estradiol-17Beta and 20Alpha-dihydro-progesterone dehydrogenase activities, within the glands and stroma, their alterations within these cell populations during the menstrual cycle, modulation of endometrial functional differentiation, in vitro, and the temporal sequence of steroid-receptor interactions will investigated. Several predictions of the scheme in regard to growth characteristics of epithelial cells and biochemical differentiation mediated by sex steroids will be followed in organ and cell culture system. Modulation of these steroid effects with inhibitors (12-0-tetradeconoyl phorbol acetate) and promotors (retinoids) of differentiation will serve to validate certain parts of the proposed scheme. Various inhibitors of transcription and translation will be utilized to indirectly demonstrate that steroid-mediated differentiation is indeed due to gene transcription. Simultaneous correlation of the biochemical end-points with the well established morphologic differentiation will help to identify the differentiation stages.

干细胞介导的分化概念解释了这一过程组织再生被提出作为循环更新的模型人类子宫内膜。类固醇激素对子宫内膜功能的调节这构成了拟议计划的主要部分，将在研究的初始阶段。因此，细胞分布激素敏感标记物，例如雌二醇和黄体酮受体和雌二醇-17Beta 和 20α-二氢孕酮脱氢酶腺体和间质内的活动，它们在这些内的改变月经周期期间的细胞群，子宫内膜的调节体外功能分化和时间序列将研究类固醇-受体相互作用。几个预测关于上皮细胞生长特性的方案和由性类固醇介导的生化分化将在器官和细胞培养系统。调节这些类固醇的作用抑制剂（12-0-十四烷酰佛波醇乙酸酯）和促进剂（类维生素A）差异化将有助于验证拟议的某些部分方案。各种转录和翻译抑制剂将被用于间接证明类固醇介导的分化是确实是由于基因转录。同时关联生化终点具有完善的形态学分化将有助于识别分化阶段。